Distribution of Virulence Factors and Resistance Determinants in Three Genotypes of Staphylococcus argenteus Clinical Isolates in Japan.
Distribution of Virulence Factors and Resistance Determinants in Three Genotypes of Staphylococcus argenteus Clinical Isolates in Japan.
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DOI：
10.3390/pathogens10020163
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发表时间：
2021-02-03
期刊：
影响因子：
--
通讯作者：
Kobayashi N
中科院分区：
文献类型：
--
作者：
Aung MS;Urushibara N;Kawaguchiya M;Hirose M;Ike M;Ito M;Kobayashi N
银葡萄球菌是一种新的葡萄球菌，独立于S。金黄色葡萄球菌引起广泛的传染病。随着世界范围内越来越多地报告从人类和动物中检测到该物种，其通过外部遗传决定因素的日益增长的毒力和耐药性已引起关注。本研究调查了82株沙门氏菌的毒力因子和耐药决定因子的流行情况和遗传特征。从2019年8月开始，在日本北海道开展为期一年的银汉滩病毒临床分离株研究。这些S.银染菌株占总菌株数的0.66%。在同一时期收集的金黄色葡萄球菌分离株。最常见的基因型是序列型（ST）2250和葡萄球菌凝固酶（coa）基因型XId（45.1%，n = 37），其次是ST 1223-coa XV（30.5%，n = 25）和ST 2198-coa XIV（24.4%，n = 20）。Panton-Valentine杀白细胞素基因（lukS-PV-lukF-PV）在单个ST 2250分离株中鉴定。仅ST 1223分离株具有肠毒素基因簇（egc-2）、seb和selw（检出率分别为100%、60%和84%），而sec、sey、sel 26-sel 27、tst-1仅在ST 2250分离株中检出（检出率分别为10.8%、100%、67.6%和10.8%）。ST 2198分离株窝藏selx在一个显着较高的利率（60%）比其他ST分离株。虽然大多数S。ST 2198对青霉素类、大环内酯类和氨基糖苷类抗生素耐药率较高，并携带blaZ、lac（C）、msr（A）、lnuA和aac（6′）-Ie-aph（2″）-Ia等多种耐药基因。只有一个ST 2250分离株具有SCCmec-IVc，显示苯唑西林耐药。blaZ是三种ST中最普遍的抗性决定因素，属于两个质粒组和一个染色体组，表明其起源不同。lnu（A）在ST 2198分离株被分配到一个主要的集群与各种葡萄球菌物种。目前的研究表明，流行的毒力因子和耐药谱/决定因素的不同取决于谱系（ST）的S。阿让提斯
Staphylococcus argenteus, a novel staphylococcal species independent of S. aureus, causes a wide spectrum of infectious diseases. As detection of this species from humans and animals has been increasingly reported worldwide, its growing virulence and drug resistance via external genetic determinants has become concerning. In this study, the prevalence and genetic characteristics of virulence factors and drug resistance determinants were investigated for 82 S. argenteus clinical isolates in Hokkaido, Japan, for a one-year period starting in August 2019. These S. argenteus isolates corresponded to 0.66% of the total number of S. aureus isolates collected in the same period. The most prevalent genotype was sequence type (ST) 2250 and staphylocoagulase (coa) genotype XId (45.1%, n = 37), followed by ST1223-coa XV (30.5%, n = 25) and ST2198-coa XIV (24.4%, n = 20). Panton-Valentine leukocidin genes (lukS-PV-lukF-PV) were identified in a single ST2250 isolate. Only ST1223 isolates had the enterotoxin gene cluster (egc-2), seb, and selw (detection rate; 100%, 60%, and 84%, respectively), while sec, sey, sel26-sel27, tst-1 were only detected in ST2250 isolates (detection rate; 10.8%, 100%, 67.6%, and 10.8%, respectively). ST2198 isolates harbored selx at a significantly higher rate (60%) than isolates of other STs. Although most of S. argenteus isolates were susceptible to antimicrobials examined, ST2198 showed higher resistance rates to penicillin, macrolides, and aminoglycosides than other STs, and it harbored various resistance genes such as blaZ, erm(C), msr(A), lnuA, and aac(6′)-Ie-aph(2″)-Ia. Only one ST2250 isolate possessed SCCmec-IVc, showing resistance to oxacillin. blaZ was the most prevalent determinant of resistance in the three STs and belonged to two plasmid groups and a chromosomal group, suggesting its diverse origin. lnu(A) in ST2198 isolates was assigned to a major cluster with various staphylococcal species. The present study indicates that the prevalence of virulence factors and drug resistance profile/determinants differ depending on the lineage (ST) of S. argenteus.
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DOI：
10.1007/s10096-020-03950-4
发表时间：
2020-12
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Alhussein F;Fürstenberg J;Gaupp R;Eisenbeis J;Last K;Becker SL;Papan C
通讯作者：
Papan C
影响因子：
5.6
作者：
Diot A;Dyon-Tafani V;Bergot M;Tasse J;Martins-Simões P;Josse J;Valour F;Laurent F
通讯作者：
Laurent F
影响因子：
5.2
作者：
Hansen TA;Bartels MD;Høgh SV;Dons LE;Pedersen M;Jensen TG;Kemp M;Skov MN;Gumpert H;Worning P;Westh H
通讯作者：
Westh H
影响因子：
4.9
作者：
Hao, Samantha;Abdelghany, Mazin;Langelier, Charles
通讯作者：
Langelier, Charles
影响因子：
2.6
作者：
Aung, Meiji Soe;Urushibara, Noriko;Kobayashi, Nobumichi
通讯作者：
Kobayashi, Nobumichi