Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus.
Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus.
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DOI：
10.1016/j.virol.2013.04.028
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发表时间：
2013-08-15
期刊：
影响因子：
3.7
通讯作者：
Dixon LK
中科院分区：
文献类型：
--
作者：
Abrams CC;Goatley L;Fishbourne E;Chapman D;Cooke L;Oura CA;Netherton CL;Takamatsu HH;Dixon LK
非洲猪瘟病毒（ASFV）引起家猪的急性出血性疾病，目前尚无有效的疫苗。减毒ASFV毒株OUR T88/3先前已显示保护接种疫苗的猪免受包括OUR T88/1在内的一些强毒株的攻击。从OUR T88/3基因组中缺失两个基因DP 71 L和DP 96 R以产生重组病毒OUR T88/3Δ DP 2。先前已经证明，从强毒病毒中删除这些基因可以降低ASFV在家猪中的毒力。用缺失病毒OUR T88/3Δ DP 2或亲本病毒OUR T88/3免疫6头猪的组，并用毒性OUR T88/1病毒攻击。4头猪（66%）通过接种缺失病毒OUR T88/3Δ DP 2得到保护，而亲本病毒OUR T88/3的保护率为100%。因此，从OUR T88/3株中缺失两个基因DP 71 L和DP 96 R降低了其保护猪抵抗强毒攻击的能力。减毒株OUR T88/3保护猪免受强毒株OUR T88/1的攻击。OUR T88/3编码毒力相关基因DP 71 L和DP 96 R。缺失DP 71 L和DP 96 R基因的重组病毒OUR T88/3Δ DP 2的构建重组病毒OUR T88/3Δ DP 2的体外生长与OUR T88/3相似。接种OUR T88/3Δ DP 2的猪在用OUR T88/1攻击时显示保护降低
African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains including OUR T88/1. Two genes, DP71L and DP96R were deleted from the OUR T88/3 genome to create recombinant virus OUR T88/3ΔDP2. Deletion of these genes from virulent viruses has previously been shown to reduce ASFV virulence in domestic pigs. Groups of 6 pigs were immunised with deletion virus OUR T88/3ΔDP2 or parental virus OUR T88/3 and challenged with virulent OUR T88/1 virus. Four pigs (66%) were protected by inoculation with the deletion virus OUR T88/3ΔDP2 compared to 100% protection with the parental virus OUR T88/3. Thus the deletion of the two genes DP71L and DP96R from OUR T88/3 strain reduced its ability to protect pigs against challenge with virulent virus. Attenuated strain OUR T88/3 protects pigs against challenge with virulent strain OUR T88/1. OUR T88/3 encodes virulence associated genes DP71L and DP96R. Construction of recombinant virus OUR T88/3ΔDP2 with DP71L and DP96R genes deleted. Growth of recombinant virus OUR T88/3ΔDP2 in vitro is similar to OUR T88/3. Pigs inoculated with OUR T88/3ΔDP2 show reduced protection when challenged with OUR T88/1
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