An engineered dimeric protein pore that spans adjacent lipid bilayers.
An engineered dimeric protein pore that spans adjacent lipid bilayers.
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DOI：
10.1038/ncomms2726
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发表时间：
2013
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
人工组织的自下而上构建是合成生物学中尚未开发的领域。一个重要的挑战是工程组织的组成区室之间以及工程组织与其他区室（包括细胞外液、进一步工程组织和活细胞）之间的连通。在这里，我们提出了一种二聚体跨膜孔，它可以跨越两个相邻的脂质双层，从而允许水室连通。两个七聚葡萄球菌 α-溶血素 (αHL) 孔以帽对帽方向共价连接。二聚体 (α7)2 的结构通过生化分析、透射电子显微镜 (TEM) 和单通道电记录得到证实。我们证明 (α7)2 的两个 β 桶之一可以插入小单层囊泡的脂质双层中，而另一个则跨越平面脂质双层。通过 TEM 还观察到跨越两个双层的 (α7)2 孔。
The bottom-up construction of artificial tissues is an underexplored area of synthetic biology. An important challenge is communication between constituent compartments of the engineered tissue and between the engineered tissue and additional compartments, including extracellular fluids, further engineered tissue and living cells. Here we present a dimeric transmembrane pore that can span two adjacent lipid bilayers and thereby allow aqueous compartments to communicate. Two heptameric staphylococcal α-hemolysin (αHL) pores were covalently linked in an aligned cap-to-cap orientation. The structure of the dimer, (α7)2, was confirmed by biochemical analysis, transmission electron microscopy (TEM) and single-channel electrical recording. We show that one of two β barrels of (α7)2 can insert into the lipid bilayer of a small unilamellar vesicle, while the other spans a planar lipid bilayer. (α7)2 pores spanning two bilayers were also observed by TEM.
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影响因子：
5.5
作者：
Hess, Berk;Kutzner, Carsten;Lindahl, Erik
通讯作者：
Lindahl, Erik
影响因子：
--
作者：
Maglia, Giovanni;Heron, Andrew J.;Stoddart, David;Japrung, Deanpen;Bayley, Hagan
通讯作者：
Bayley, Hagan
影响因子：
8
作者：
Kleinschmidt, JH;Wiener, MC;Tamm, LK
通讯作者：
Tamm, LK
DOI：
10.1073/pnas.91.26.12828
发表时间：
1994-12-20
影响因子：
11.1
作者：
GOUAUX, JE;BRAHA, O;BAYLEY, H
通讯作者：
BAYLEY, H
影响因子：
21.8
作者：
Kurihara, Kensuke;Tamura, Mieko;Sugawara, Tadashi
通讯作者：
Sugawara, Tadashi