Host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth.
Host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth.
复制标题
DOI：
10.1186/s40168-017-0384-1
复制
发表时间：
2018-01-04
期刊：
影响因子：
15.5
通讯作者：
Maltecca C
中科院分区：
文献类型：
--
作者：
Lu D;Tiezzi F;Schillebeeckx C;McNulty NP;Schwab C;Shull C;Maltecca C
在猪中，肠道细菌已被证明在宿主的营养、生理和免疫过程中发挥重要作用。然而，它们的宏基因组或其中的一部分的贡献，这通常反映了16 S rRNA编码基因的片段，尚未得到充分的研究。在三个时间点，包括断奶、断奶后第15周（下文称为“第15周”）和喂养结束测试（下文称为“停止测试”），从杂交猪群体收集的粪便样品用于评估每只动物的粪便微生物组的组成随时间的变化。本研究分别使用了在断奶、第15周和试验结束时采集的1205、1295和1283份样本。有1039只动物在所有三个时间点采集了样品，并且还具有背脂厚度（BF）和平均日增重（ADG）的表型记录。厚壁菌门和拟杆菌门在所有三个时间点都是最丰富的门。在所有三个时间点最丰富的属包括梭菌属、埃希氏菌属、拟杆菌属、普雷沃氏菌属、瘤胃球菌属、梭杆菌属、弯曲杆菌属、真杆菌属和乳杆菌属。在每个时间点鉴定两种肠型。然而，只有第15周和试验结束时的肠型与BF显著相关。我们在此报告两个新的发现：（i）α多样性和操作分类单位（OTU）丰富度在第15周时以及在非测试时是中等可遗传的，h2分别为0.15 ± 0.06至0.16 ± 0.07和0.23 ± 0.09至0.26 ± 0.08，h2分别为0.20 ± 0.09 ~ 0.33 ± 0.10和0.17 ± 0.08 ~ 0.24 ± 0.08，而在断奶时检测到这两个指标的遗传力估计值非常低;和（ii）第15周的α多样性与BF（-0.53 ± 0.23至-0.45 ± 0.25）以及ADG具有很强的负遗传相关性，− 0.53 ± 0.32至− 0.53 ± 0.29。这些结果对于遗传改良家猪的努力非常重要，因为它们表明粪便微生物群多样性可以用作指标性状，以改善测量成本高的性状。本文的在线版本（10.1186/s40168-017-0384-1）包含补充材料，可供授权用户使用。
In pigs, gut bacteria have been shown to play important roles in nutritional, physiological, and immunological processes in the host. However, the contribution of their metagenomes or part of them, which are normally reflected by fragments of 16S rRNA-encoding genes, has yet to be fully investigated. Fecal samples, collected from a population of crossbred pigs at three time points, including weaning, week 15 post weaning (hereafter “week 15”), and end-of-feeding test (hereafter “off-test”), were used to evaluate changes in the composition of the fecal microbiome of each animal over time. This study used 1205, 1295, and 1283 samples collected at weaning, week 15, and off-test, respectively. There were 1039 animals that had samples collected at all three time points and also had phenotypic records on back fat thickness (BF) and average daily body weight gain (ADG). Firmicutes and Bacteroidetes were the most abundant phyla at all three time points. The most abundant genera at all three time points included Clostridium, Escherichia, Bacteroides, Prevotella, Ruminococcus, Fusobacterium, Campylobacter, Eubacterium, and Lactobacillus. Two enterotypes were identified at each time point. However, only enterotypes at week 15 and off-test were significantly associated with BF. We report herein two novel findings: (i) alpha diversity and operational taxonomic unit (OTU) richness were moderately heritable at week 15, h2 of 0.15 ± 0.06 to 0.16 ± 0.07 and 0.23 ± 0.09 to 0.26 ± 0.08, respectively, as well as at off-test, h2 of 0.20 ± 0.09 to 0.33 ± 0.10 and 0.17 ± 0.08 to 0.24 ± 0.08, respectively, whereas very low heritability estimates for both measures were detected at weaning; and (ii) alpha diversity at week 15 had strong and negative genetic correlations with BF, − 0.53 ± 0.23 to − 0.45 ± 0.25, as well as with ADG, − 0.53 ± 0.32 to − 0.53 ± 0.29. These results are important for efforts to genetically improve the domesticated pig because they suggest fecal microbiota diversity can be used as an indicator trait to improve traits that are expensive to measure. The online version of this article (10.1186/s40168-017-0384-1) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1017/s175173111600286x
发表时间：
2017-09
期刊：
Animal : an international journal of animal bioscience
影响因子：
--
作者：
Gilbert H;Billon Y;Brossard L;Faure J;Gatellier P;Gondret F;Labussière E;Lebret B;Lefaucheur L;Le Floch N;Louveau I;Merlot E;Meunier-Salaün MC;Montagne L;Mormede P;Renaudeau D;Riquet J;Rogel-Gaillard C;van Milgen J;Vincent A;Noblet J
通讯作者：
Noblet J
影响因子：
3.6
作者：
Fouhse, J. M.;Zijlstra, R. T.;Willing, B. P.
通讯作者：
Willing, B. P.
影响因子：
3.3
作者：
Hager, Reinmar;Johnstone, Rufus A.
通讯作者：
Johnstone, Rufus A.