Allelic variants of a potato HEAT SHOCK COGNATE 70 gene confer improved tuber yield under a wide range of environmental conditions.
Allelic variants of a potato HEAT SHOCK COGNATE 70 gene confer improved tuber yield under a wide range of environmental conditions.
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DOI：
10.1002/fes3.377
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发表时间：
2023-01
影响因子：
5
通讯作者：
中科院分区：
文献类型：
--
作者：
在此之前，我们开发并应用了热胁迫下马铃薯块茎产量的温室筛选，并通过对一个二倍体马铃薯群体的遗传分析，确定了一个耐热性候选基因（HSc70）。由于基因启动子序列的变化，特定的等位基因变异在中等高温下高水平表达。在这项研究中，我们的目标是在包括肯尼亚、马拉维和英国在内的几个季节和地点进行的田间试验中确认温室筛选的结果。我们扩展了对HSc70基因的理解，并证明了HSc70的表达水平与广泛的野生马铃薯近缘种的耐热性相关。我们探索了HSc70保护作用的生理基础，发现携带高表达HSc70 A2等位基因的基因型可以保护PSII免受非生物胁迫诱导的光氧化损伤。总的来说，我们展示了HSc70等位基因在多种环境下培育弹性马铃薯基因型的潜力。马铃薯是粮食安全的重要作物；然而，即使在适度升高的温度下，它也容易降低块茎产量。在这项工作中，我们展示了HSc70等位基因在多种环境下培育弹性马铃薯基因型的潜力。
Previously, we developed and applied a glasshouse screen for potato tuber yield under heat stress and identified a candidate gene (HSc70) for heat tolerance by genetic analysis of a diploid potato population. Specific allelic variants were expressed at high levels on exposure to moderately elevated temperature due to variations in gene promoter sequence. In this study, we aimed to confirm the results from the glasshouse screen in field trials conducted over several seasons and locations including those in Kenya, Malawi and the UK. We extend our understanding of the HSc70 gene and demonstrate that expression level of HSc70 correlates with tolerance to heat stress in a wide range of wild potato relatives. The physiological basis of the protective effect of HSc70 was explored and we show that genotypes carrying the highly expressed HSc70 A2 allele are protected against photooxidative damage to PSII induced by abiotic stresses. Overall, we show the potential of HSc70 alleles for breeding resilient potato genotypes for multiple environments. Potato is a critical crop for food security; however, it is vulnerable to reduced tuber yield at even moderately elevated temperature. In this work, we show the potential of HSc70 alleles for breeding resilient potato genotypes for multiple environments.
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DOI：
10.1534/g3.118.200377
发表时间：
2018-10-03
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Sharma SK;MacKenzie K;McLean K;Dale F;Daniels S;Bryan GJ
通讯作者：
Bryan GJ
通过温度依赖的热震等位基因同源70的特定等位基因的温度依赖性表达在马铃薯中的工程热耐受性。
影响因子：
13.8
作者：
Trapero-Mozos A;Morris WL;Ducreux LJM;McLean K;Stephens J;Torrance L;Bryan GJ;Hancock RD;Taylor MA
通讯作者：
Taylor MA
影响因子：
9.2
作者：
Pham GM;Hamilton JP;Wood JC;Burke JT;Zhao H;Vaillancourt B;Ou S;Jiang J;Buell CR
通讯作者：
Buell CR
影响因子：
1.5
作者：
Guedes, Marcio L.;Haynes, Kathleen G.;Pinto, Cesar A. B. P.
通讯作者：
Pinto, Cesar A. B. P.
影响因子：
4.3
作者：
Campos, PS;Quartin, V;Nunes, MA
通讯作者：
Nunes, MA