Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of HEAT-SHOCK COGNATE 70.
Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of HEAT-SHOCK COGNATE 70.
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通过温度依赖的热震等位基因同源70的特定等位基因的温度依赖性表达在马铃薯中的工程热耐受性。
DOI：
10.1111/pbi.12760
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发表时间：
2018-01
影响因子：
13.8
通讯作者：
Taylor MA
中科院分区：
文献类型：
--
作者：
Trapero-Mozos A;Morris WL;Ducreux LJM;McLean K;Stephens J;Torrance L;Bryan GJ;Hancock RD;Taylor MA
对于许多商业马铃薯栽培品种，块茎产量在14-22 °C范围内的平均日间温度下最佳。环境温度的进一步升高会减少或完全抑制马铃薯块茎的生产，对生产者和消费者都有破坏性后果。本研究的目的是使用一个遗传筛选的基础上的模型结薯测定，以确定数量性状基因座（QTL）与提高块茎产量。在一个马铃薯二倍体杂种群体（06 H1）中，在与产量提高相关的三个QTL区间之一内鉴定到编码HSc 70的候选基因。一个特定的HSc 70等位基因变异体与06 H1后代中的产量提高有关。该等位基因变体的表达比其他等位基因高得多，特别是在暴露于适度升高的温度时。该等位基因在本氏烟草中的瞬时表达导致了对高温的耐受性显着增强。TA重复元件存在于该等位基因的启动子中，但不存在于人群中鉴定的其他HSc 70等位基因中。在马铃薯栽培品种Desiree中在其天然启动子下表达HSc 70等位基因变体导致在升高的温度下增强的HSc 70表达。这反映在更大的耐受性，以确定在适度升高的温度下，在一个模型节切割块茎化系统和从茎扦插生长的植物的产量提高热应力。我们的研究结果确定HSc 70的表达水平作为一个重要的因素，影响产量稳定性在适度升高的温度和确定特定的等位基因变体的HSc 70诱导耐热性通过传统的基因渗入或分子育种方法。
For many commercial potato cultivars, tuber yield is optimal at average daytime temperatures in the range of 14–22 °C. Further rises in ambient temperature can reduce or completely inhibit potato tuber production, with damaging consequences for both producer and consumer. The aim of this study was to use a genetic screen based on a model tuberization assay to identify quantitative trait loci (QTL) associated with enhanced tuber yield. A candidate gene encoding HSc70 was identified within one of the three QTL intervals associated with elevated yield in a Phureja–Tuberosum hybrid diploid potato population (06H1). A particular HSc70 allelic variant was linked to elevated yield in the 06H1 progeny. Expression of this allelic variant was much higher than other alleles, particularly on exposure to moderately elevated temperature. Transient expression of this allele in Nicotiana benthamiana resulted in significantly enhanced tolerance to elevated temperature. An TA repeat element was present in the promoter of this allele, but not in other HSc70 alleles identified in the population. Expression of the HSc70 allelic variant under its native promoter in the potato cultivar Desiree resulted in enhanced HSc70 expression at elevated temperature. This was reflected in greater tolerance to heat stress as determined by improved yield under moderately elevated temperature in a model nodal cutting tuberization system and in plants grown from stem cuttings. Our results identify HSc70 expression level as a significant factor influencing yield stability under moderately elevated temperature and identify specific allelic variants of HSc70 for the induction of thermotolerance via conventional introgression or molecular breeding approaches.
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影响因子：
4.3
作者：
Campos, PS;Quartin, V;Nunes, MA
通讯作者：
Nunes, MA
影响因子：
64.8
作者：
Kloosterman, Bjorn;Abelenda, Jose A.;Bachem, Christian W. B.
通讯作者：
Bachem, Christian W. B.
影响因子：
9.4
作者：
KUMAR, D;WAREING, PF
通讯作者：
WAREING, PF
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
6.9
作者：
Ducreux LJ;Morris WL;Prosser IM;Morris JA;Beale MH;Wright F;Shepherd T;Bryan GJ;Hedley PE;Taylor MA
通讯作者：
Taylor MA