Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules.
Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules.
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DOI：
10.1016/j.cell.2008.09.046
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发表时间：
2008-11-28
期刊：
影响因子：
64.5
通讯作者：
Odde DJ
中科院分区：
文献类型：
--
作者：
Gardner MK;Bouck DC;Paliulis LV;Meehl JB;O'Toole ET;Haase J;Soubry A;Joglekar AP;Winey M;Salmon ED;Bloom K;Odde DJ
During mitosis, sister chromatids congress to the spindle equator and are subsequently segregated via attachment to dynamic kinetochore microtubule (kMT) plus-ends. A major question is how kMT plus-end assembly is spatially regulated to achieve chromosome congression. Here we find in budding yeast that the widely-conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kMTs. Of the two, Cin8p is the major effector and its activity requires a functional motor domain. In contrast, the depolymerizing kinesin-8 motor Kip3p plays a minor role in spatial regulation of yeast kMT assembly. Our analysis identified a model where kinesin-5 motors bind to kMTs, move to kMT plus ends, and upon arrival at a growing plus-end promote net kMT plus-end disassembly. In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression.
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DOI：
10.1083/jcb.138.3.629
发表时间：
1997-08-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carminati JL;Stearns T
通讯作者：
Stearns T
DOI：
10.1083/jcb.200606021
发表时间：
2006-10-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang B;Huffaker TC
通讯作者：
Huffaker TC
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
通讯作者：
Saunders WS
影响因子：
21.3
作者：
Gupta, Mohan L., Jr.;Carvalho, Pedro;Pellman, David
通讯作者：
Pellman, David
影响因子：
4.3
作者：
de Gramont, Armand;Barbour, Leslie;Cohen-Fix, Orna
通讯作者：
Cohen-Fix, Orna