N-Acetylgalactosaminyltransferase-14 as a potential biomarker for breast cancer by immunohistochemistry.
N-Acetylgalactosaminyltransferase-14 as a potential biomarker for breast cancer by immunohistochemistry.
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N-乙酰半乳糖胺基转移酶-14 通过免疫组织化学作为乳腺癌的潜在生物标志物
DOI：
10.1186/1471-2407-10-123
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发表时间：
2010-04-01
期刊：
影响因子：
3.8
通讯作者：
Zhu M
中科院分区：
文献类型：
--
作者：
Wu C;Guo X;Wang W;Wang Y;Shan Y;Zhang B;Song W;Ma S;Ge J;Deng H;Zhu M
背景蛋白质的翻译后修饰，包括糖基化，在正常和肿瘤细胞中是不同的。UDP-N-乙酰基-D-半乳糖胺多肽N-乙酰基半乳糖胺转移酶（GalNAc-Tases）家族的酶调节粘蛋白0-糖基化的初始步骤，并且负责在癌细胞中观察到的改变的糖基化状态。最近发现，GalNAc-T14 mRNA在乳腺癌中与正常组织相比不均匀地表达，然而GalNAc-T14蛋白在乳腺癌中与正常组织相比的表达谱仍然未知。在这项研究中，我们评估了GalNAc-T14蛋白在恶性和非恶性乳腺组织中的表达谱，通过免疫组织化学，以评估是否GalNAc-T14可能是一个潜在的生物标志物乳腺cancer.MethodsIn福尔马林固定的组织中，GalNAc-T14蛋白的表达水平进行了评估，在乳腺组织中通过免疫组织化学方法。在正常组织，良性纤维腺瘤和几种类型的carcinomas.ResultsOur结果表明，GalNAc-T14的表达谱进行了评估，在乳腺癌中的异质性表达相比，非恶性组织。GalNAc-T14在47/56（83.9%）的癌组织和7/48（14.6%）的非恶性乳腺组织中表达。GalNAc-T14表达水平与组织学分级相关。对于这种酶与浸润性导管型，粘液腺癌和导管原位癌（DCIS）的类型的显着关联被发现。ConclusionOur结果提供的证据表明，GalNAc-T14可能是一个潜在的生物标志物乳腺癌免疫组化。GalNAc-T14表达水平与组织学分级相关。GalNAc-T14的表达可以为乳腺癌糖生物学提供新的见解。
BackgroundThe post-translational modification of proteins, including glycosylation, differs between normal and tumor cells. The UDP-N-acetyl-D-galactosamine polypeptide N-acetylgalactosaminyltransferases (GalNAc-Tases) family of enzymes regulates the initial steps of mucin O-glycosylation and is responsible for the altered glycosylation state observed in cancer cells. Recently it was found that GalNAc-T14 mRNA is heterogeneously expressed in breast carcinomas compared to normal tissue, however the expression profile of GalNAc-T14 protein in breast carcinomas compared to normal tissue is still unknown. In this study, we assessed the expression profile of GalNAc-T14 protein in malignant and non-malignant breast tissues by immunohistochemistry to evaluate whether GalNAc-T14 might be a potential biomarker for breast cancer.MethodsIn formalin-fixed tissues, the expression level of GalNAc-T14 protein was evaluated by immunohistochemistry assay in breast tissues. Expression profiles were assessed in normal tissues, benign fibroadenomas and several types of carcinomas.ResultsOur results showed that GalNAc-T14 was heterogeneously expressed in breast carcinomas compared to non-malignant tissue. GalNAc-T14 expression was observed in 47/56 (83.9%) carcinoma samples, 7/48 (14.6%) non-malignant breast tissue samples. GalNAc-T14 expression level was associated with histological grade. For this enzyme a significant association with invasive ductal type, mucinous adenocarcinoma and ductal carcinoma in situ (DCIS) type was found.ConclusionOur results provide evidence that GalNAc-T14 may be a potential biomarker for breast cancer by immunohistochemistry. GalNAc-T14 expression level was associated with histological grade. GalNAc-T14 expression can provide new insights about breast cancer glycobiology.
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影响因子：
4.3
作者：
Mandel, U;Hassan, H;Clausen, H
通讯作者：
Clausen, H
DOI：
10.1016/0304-419x(94)90002-7
发表时间：
1994-05-27
影响因子：
11.2
作者：
PONTA, H;SLEEMAN, J;HERRLICH, P
通讯作者：
HERRLICH, P
影响因子：
11.5
作者：
Miyahara, N;Shoda, J;Irimura, T
通讯作者：
Irimura, T
影响因子：
3.5
作者：
Bennett, EP;Hassan, H;Clausen, H
通讯作者：
Clausen, H
影响因子：
4.8
作者：
Bennett, EP;Hassan, H;Clausen, H
通讯作者：
Clausen, H