Postmortem changes in the neuroanatomical characteristics of the primate brain: hippocampal formation.
Postmortem changes in the neuroanatomical characteristics of the primate brain: hippocampal formation.
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DOI：
10.1002/cne.21906
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发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Amaral, David G.
中科院分区：
文献类型：
--
作者：
Lavenex, Pierre;Lavenex, Pamela Banta;Bennett, Jeffrey L.;Amaral, David G.
关键词：
大脑结构组织的比较研究是我们理解人脑功能的基础。然而，尽管实验动物的大脑是通过血管系统灌注固定剂来固定的，但人类或猿的大脑是在不同的死后间隔后通过浸泡来固定的。虽然不同的治疗可能会影响组织的基本特征，这个问题还没有在灵长类动物的大脑经验评估。猴脑灌注，或收购后，不同的死后间隔，浸泡固定在4%多聚甲醛。我们发现，固定方法影响猴海马结构的神经解剖学特征。与灌注固定的组织相比，尽管总体脑体积较大，但在Nissl染色的浸没固定的组织中索马尺寸较小。非磷酸化的高分子量神经丝免疫反应性较低，在CA 3锥体神经元，齿状苔藓细胞和内嗅皮质，而它是较高的苔藓纤维途径浸泡固定的组织。5-羟色胺免疫反应纤维染色良好，灌注组织，但在浸泡固定的组织中检测不到。虽然区域的钙结合蛋白的免疫反应性模式没有受到影响，细胞内染色降解增加死后间隔。在浸没固定的猴组织中观察到生长抑素免疫反应簇的大轴突静脉曲张，以前只在人类中报道。此外，calretinin免疫反应多极神经元，以前只在啮齿动物中观察到，被发现在嘴侧齿状回灌注和浸泡固定的大脑。总之，大脑的比较研究必须在得出物种差异的结论之前，评估固定对每个感兴趣结构中每个标记物染色模式的影响。
Comparative studies of the structural organization of the brain are fundamental to our understanding of human brain function. However, whereas brains of experimental animals are fixed by perfusion of a fixative through the vasculature, human or ape brains are fixed by immersion after varying postmortem intervals. Although differential treatments might affect the fundamental characteristics of the tissue, this question has not been evaluated empirically in primate brains. Monkey brains were either perfused, or acquired after varying postmortem intervals before immersion-fixation in 4% paraformaldehyde. We found that the fixation method affected the neuroanatomical characteristics of the monkey hippocampal formation. Soma size was smaller in Nissl-stained, immersion-fixed tissue, although overall brain volume was larger, as compared to perfusion-fixed tissue. Non-phosphorylated high-molecular-weight neurofilament immunoreactivity was lower in CA3 pyramidal neurons, dentate mossy cells and the entorhinal cortex, whereas it was higher in the mossy fiber pathway in immersion-fixed tissue. Serotonin-immunoreactive fibers were well-stained in perfused tissue but were undetectable in immersion-fixed tissue. Although regional immunoreactivity patterns for calcium-binding proteins were not affected, intracellular staining degraded with increasing postmortem intervals. Somatostatin-immunoreactive clusters of large axonal varicosities, previously reported only in humans, were observed in immersion-fixed monkey tissue. In addition, calretinin-immunoreactive multipolar neurons, previously observed only in rodents, were found in the rostral dentate gyrus in both perfused and immersion-fixed brains. In conclusion, comparative studies of the brain must evaluate the effects of fixation on the staining pattern of each marker in every structure of interest before drawing conclusions about species differences.
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影响因子：
3.3
作者：
Altemus, KL;Lavenex, P;Amaral, DG
通讯作者：
Amaral, DG
影响因子：
2.5
作者：
AMARAL, DG;INSAUSTI, R;COWAN, WM
通讯作者：
COWAN, WM
影响因子：
3.3
作者：
Brady, DR;Mufson, EJ
通讯作者：
Mufson, EJ
影响因子：
3.3
作者：
GULYAS, AI;MIETTINEN, R;FREUND, TF
通讯作者：
FREUND, TF
影响因子：
2.5
作者：
AMARAL, DG;INSAUSTI, R;COWAN, WM
通讯作者：
COWAN, WM