Alcohol-induced changes in opioid peptide levels in adolescent rats are dependent on housing conditions.
Alcohol-induced changes in opioid peptide levels in adolescent rats are dependent on housing conditions.
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DOI：
10.1111/acer.12586
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Nylander I
中科院分区：
文献类型：
--
作者：
Palm S;Nylander I
内源性阿片类物质与酒精的作用机制有关，酒精会影响阿片类物质在许多大脑区域的作用，尽管人们对酒精对青少年大脑中阿片类物质的影响知之甚少。一个问题，特别是在研究年轻动物时，是酒精摄入模型往往是基于单一的住房，可能会导致酒精的影响混淆缺乏社会互动。本研究的目的是调查短期和长期酒精对阿片类药物的影响以及住房条件对这些影响的影响。在第一部分中，在青少年和成年大鼠中，在一次24小时的单独饲养和2小时的自愿酒精摄入后测量阿片肽水平。在第二部分中，测试了在2小时饮酒期间插入笼分隔器的模型，并在青少年自愿摄入阿片类药物6周后，分别在单对和成对饲养的大鼠中检查了对阿片类药物的影响。单一住房的影响是年龄特异性的，特别是影响甲硫氨酸-脑啡肽-Arg 6Phe 7（MEAP）。在青春期大鼠中，很难区分酒精和单一住房引起的影响，而在成年大鼠中，在强啡肽B（DYNB）、β-内啡肽（弯曲）和MEAP水平中观察到酒精特异性影响。自愿饮酒影响了几个大脑区域，大多数酒精引起的影响并不依赖于住房。然而，酒精对杏仁核中DYNB和弯曲的影响依赖于住房。住房单独影响MEAP在扣带皮层。此外，在不同的住房条件下，长期自愿饮酒产生了几种独立于住房的酒精诱导效应。然而，在涉及压力、情绪和酒精使用障碍的区域发现了住房依赖效应。因此，住房条件和年龄可能会影响饮酒的原因和潜在机制，并可能影响酒精摄入量研究的一些终点结果。
Endogenous opioids are implicated in the mechanism of action of alcohol and alcohol affects opioids in a number of brain areas, although little is known about alcohol's effects on opioids in the adolescent brain. One concern, in particular when studying young animals, is that alcohol intake models often are based on single housing that may result in alcohol effects confounded by the lack of social interactions. The aim of this study was to investigate short- and long-term alcohol effects on opioids and the influence of housing conditions on these effects. In the first part, opioid peptide levels were measured after one 24-hour session of single housing and 2-hour voluntary alcohol intake in adolescent and adult rats. In the second part, a model with a cage divider inserted during 2-hour drinking sessions was tested and the effects on opioids were examined after 6 weeks of adolescent voluntary intake in single-and pair-housed rats, respectively. The effects of single housing were age specific and affected Met-enkephalin-Arg6Phe7 (MEAP) in particular. In adolescent rats, it was difficult to distinguish between effects induced by alcohol and single housing, whereas alcohol-specific effects were seen in dynorphin B (DYNB), beta-endorphin (BEND), and MEAP levels in adults. Voluntary drinking affected several brain areas and the majority of alcohol-induced effects were not dependent on housing. However, alcohol effects on DYNB and BEND in the amygdala were dependent on housing. Housing alone affected MEAP in the cingulate cortex. Age-specific housing- and alcohol-induced effects on opioids were found. In addition, prolonged voluntary alcohol intake under different housing conditions produced several alcohol-induced effects independent of housing. However, housing-dependent effects were found in areas implicated in stress, emotionality, and alcohol use disorder. Housing condition and age may therefore affect the reasons and underlying mechanisms for drinking and could potentially affect the outcome of a number of end points in research on alcohol intake.
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影响因子：
10.6
作者：
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影响因子：
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影响因子：
2.9
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Mendez, M.;Barbosa-Luna, I. G.;Oikawa, J.
通讯作者：
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影响因子：
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作者：
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通讯作者：
BARCOMB, A