The indolic diet-derivative, 3,3'-diindolylmethane, induced apoptosis in human colon cancer cells through upregulation of NDRG1.
The indolic diet-derivative, 3,3'-diindolylmethane, induced apoptosis in human colon cancer cells through upregulation of NDRG1.
复制标题
DOI：
10.1155/2012/256178
复制
发表时间：
2012
影响因子：
--
通讯作者：
Fares F
中科院分区：
文献类型：
--
作者：
Lerner A;Grafi-Cohen M;Napso T;Azzam N;Fares F
N-myc下游调节基因1参与肿瘤发生、血管生成、转移和抗癌药物耐药性。在本研究中，我们分析了N-myc下游调节基因-1在人结肠癌细胞系HCT-116（与野生型p53基因分化良好）和科洛-320（与突变型p53基因分化不良）用3，3 ′-二吲哚甲烷（一种来自吲哚-3-甲醇的成熟促凋亡剂产物）处理后的表达模式。用3，3 ′-二吲哚基甲烷处理科洛-320和HCT-116显示通过诱导凋亡以剂量依赖性方式抑制细胞活力。N-myc下游调节基因-1仅在低分化结肠癌细胞株科洛-320中表达增高。我们的研究结果表明，N-myc下游调节基因-1的表达在低分化细胞中被3，3 ′-二吲哚甲烷增强，随后诱导凋亡。3，3 ′-二吲哚甲烷诱导的低分化结肠癌细胞凋亡可能是N-myc下游调节基因-1的一种新的调控因子。
N-myc downstream regulated gene-1 participates in carcinogenesis, angiogenesis, metastases, and anticancer drug resistance. In the present study, we analyzed the expression pattern of N-myc downstream regulated gene-1 following treatment of human colonic cancer cell lines; HCT-116 (well differentiated with wild-type p53 gene) and Colo-320 (poorly differentiated with mutant p53 gene), with 3,3′-diindolylmethane, a well-established proapoptotic agent product derived from indole-3-carbinol. Treatment of Colo-320 and HCT-116 with 3,3′-diindolylmethane disclosed inhibition of cell viability in a dose-dependent manner, mediated through apoptosis induction. The increased expression of N-myc downstream regulated gene-1 was detected only in poorly differentiated colon cancer cells, Colo-320 cell line. Our results suggest that N-myc downstream regulated gene-1 expression is enhanced by 3,3′-diindolylmethane in poorly differentiated cells and followed by induction of apoptosis. 3,3′-diindolylmethane induced apoptosis may represent a new regulator of N-myc downstream regulated gene-1 in poorly differentiated colonic cancer cells.
登录
查看更多内容
影响因子：
2.6
作者：
Ando, T.;Ishiguro, H.;Kuwabara, Y.
通讯作者：
Kuwabara, Y.
胃癌早期检测和预后评估的新型分子标志物：N-myc下游调节基因1（NDRG1）
影响因子：
1.9
作者：
Jiang, Kewei;Shen, Zhanlong;Wang, Shan
通讯作者：
Wang, Shan
影响因子：
4.2
作者：
Chen, DZ;Qi, M;Carter, TH
通讯作者：
Carter, TH
影响因子：
11.5
作者：
Shah, MA;Kemeny, N;Schwartz, GK
通讯作者：
Schwartz, GK
影响因子：
3.5
作者：
Ulrix, W;Swinnen, JV;Verhoeven, G
通讯作者：
Verhoeven, G