Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes.
Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes.
复制标题
DOI：
10.1016/j.xpro.2021.100859
复制
发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Wu JC
中科院分区：
文献类型：
--
作者：
Zhang JZ;Zhao SR;Tu C;Pang P;Zhang M;Wu JC
已经开发了多种策略来有效地分化人诱导的多能干细胞衍生的心肌细胞（hiPSC-CM）。在这里，我们描述了一个协议，用于测量hiPSC-CM的三个关键功能参数，包括收缩功能，钙（Ca 2+）处理，和动作电位。有关本方案使用和执行的完整详细信息，请参阅。hiPSC-CM收缩功能的自动测量负载有Fura-2 AM的hiPSC-CM的单细胞钙成像hiPSC-CM的基于成像的动作电位测量已经开发了多种策略来有效地分化人诱导的多能干细胞衍生的心肌细胞（hiPSC-CM）。在这里，我们描述了一个协议，用于测量hiPSC-CM的三个关键功能参数，包括收缩功能，钙（Ca 2+）处理，和动作电位。
Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential. For complete details on the use and execution of this protocol, please refer to. Automated measurement of hiPSC-CM contractile function Single-cell calcium imaging of hiPSC-CMs loaded with Fura-2 AM Imaging-based action potential measurement of hiPSC-CMs Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential.
登录
查看更多内容
影响因子：
37.8
作者：
Kitani, Tomoya;Ong, Sang-Ging;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
23.9
作者：
Zhang, Joe Z.;Termglinchan, Vittavat;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
--
作者：
Lyra-Leite DM;Fonoudi H;Gharib M;Burridge PW
通讯作者：
Burridge PW
影响因子：
6.1
作者：
Greensmith DJ
通讯作者：
Greensmith DJ
影响因子：
8
作者：
Chour, Tony;Tian, Lei;Wu, Joseph C.
通讯作者：
Wu, Joseph C.