Nesfatin-1 activates cardiac vagal neurons of nucleus ambiguus and elicits bradycardia in conscious rats.
Nesfatin-1 activates cardiac vagal neurons of nucleus ambiguus and elicits bradycardia in conscious rats.
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DOI：
10.1111/jnc.12355
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发表时间：
2013-09
影响因子：
4.7
通讯作者：
Brailoiu E
中科院分区：
文献类型：
--
作者：
Brailoiu GC;Deliu E;Tica AA;Rabinowitz JE;Tilley DG;Benamar K;Koch WJ;Brailoiu E
Nesfatin-1是一种其受体尚未鉴定的肽，已参与调节摄食、应激和代谢反应。最近，越来越多的证据支持nesfatin-1在自主神经和心血管活动中的调节作用。这项研究是为了测试，如果nesfatin-1的表达在疑核，副交感神经心脏控制的关键网站，可能与功能的作用。由于我们以前已经证明，nesfatin-1 elebrants Ca 2+信号在下丘脑神经元，我们首先评估了这种肽对胞质Ca 2+的心脏神经节前疑核神经元的影响。我们提供的证据表明，nesfatin-1通过Gi/o-偶联机制增加胞浆Ca 2+浓度。nesfatin-1诱导的Ca ~（2+）升高主要依赖于P/Q型电压激活Ca ~（2+）通道的Ca ~（2+）内流。重复施用nesfatin-1导致快速耐受。此外，nesfatin通过Gi/o偶联机制产生心脏迷走神经元的剂量依赖性去极化。在体内研究中，使用遥测和尾袖监测心率和血压，表明微注射nesfatin-1到疑核产生心动过缓不伴有清醒大鼠血压的变化。总之，我们的研究结果首次确定nesfatin-1通过激活疑核的心脏迷走神经元来降低心率。
Nesfatin-1, a peptide whose receptor is yet to be identified, has been involved in the modulation of feeding, stress and metabolic responses. More recently, increasing evidence supports a modulatory role for nesfatin-1 in autonomic and cardiovascular activity. This study was undertaken to test if the expression of nesfatin-1 in the nucleus ambiguus, a key site for parasympathetic cardiac control, may be correlated with a functional role. Since we have previously demonstrated that nesfatin-1 elicits Ca2+ signaling in hypothalamic neurons, we first assessed the effect of this peptide on cytosolic Ca2+ in cardiac preganglionic neurons of nucleus ambiguus. We provide evidence that nesfatin-1 increases cytosolic Ca2+ concentration via a Gi/o-coupled mechanism. The nesfatin-1-induced Ca2+ rise is critically dependent on Ca2+ influx via P/Q-type voltage-activated Ca2+ channels. Repeated administration of nesfatin-1 leads to tachyphylaxis. Further, nesfatin produces a dose-dependent depolarization of cardiac vagal neurons via a Gi/o-coupled mechanism. In vivo studies, using telemetric and tail-cuff monitoring of heart rate and blood pressure, indicate that microinjection of nesfatin-1 into the nucleus ambiguus produces bradycardia not accompanied by a change in blood pressure in conscious rats. Taken together, our results identify for the first time that nesfatin-1 decreases heart rate by activating cardiac vagal neurons of nucleus ambiguus.
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影响因子：
3.3
作者：
Gray, AL;Johnson, TA;Massari, VJ
通讯作者：
Massari, VJ
DOI：
10.1046/j.1440-1681.2001.03531.x
发表时间：
2001-10-01
影响因子：
2.9
作者：
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通讯作者：
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影响因子：
4.7
作者：
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通讯作者：
Brailoiu E
DOI：
10.1152/ajpregu.00224.2012
发表时间：
2012-11-01
影响因子：
2.8
作者：
Chitravanshi, Vineet C.;Kawabe, Kazumi;Sapru, Hreday N.
通讯作者：
Sapru, Hreday N.
小鼠大脑中nesfatin-1神经元的定位及其功能意义。
影响因子：
2.9
作者：
Goebel-Stengel M;Wang L;Stengel A;Taché Y
通讯作者：
Taché Y