Localization of nesfatin-1 neurons in the mouse brain and functional implication.
Localization of nesfatin-1 neurons in the mouse brain and functional implication.
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小鼠大脑中nesfatin-1神经元的定位及其功能意义。
DOI：
10.1016/j.brainres.2011.04.031
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发表时间：
2011-06-17
期刊：
影响因子：
2.9
通讯作者：
Taché Y
中科院分区：
文献类型：
--
作者：
Goebel-Stengel M;Wang L;Stengel A;Taché Y
Nesfatin-1 在啮齿动物体内注射时会减少食物摄入量。我们最近描述了核结合素 2 (NUCB2)/nesfatin-1 免疫反应性在各种应激源激活的大鼠脑自主神经核中的广泛分布。我们使用 C57BL/6 小鼠定位大脑 NUCB2/nesfatin-1 免疫反应性，并评估回避应激 (WAS) 后 NUCB2/nesfatin 1 神经元的激活。还测定了非营养液体的胃排空。在皮质区域（包括梨状皮层、岛叶皮层、扣带皮层和躯体运动皮层）、边缘系统（包括杏仁核、海马和隔膜）、基底神经节、终纹床核、丘脑（包括室旁和束旁核）、下丘脑（包括视上、室周、室旁（PVN））、弓状核以及下丘脑腹内侧和外侧区域。在先前未定义的区域中检测到强烈标记的 NUCB2/nesfatin-1 神经元，我们将其命名为中间背内侧下丘脑。在脑干中，中缝核、Edinger-Westphal 核、蓝斑 (LC)、外侧臂旁核、腹外侧延髓 (VLM) 和背迷走神经复合体中检测到 NUCB2/nesfatin-1 免疫反应性。 WAS 在 PVN 中 35% 的 NUCB2/nesfatin-1 免疫反应性神经元、LC 中 50%、中缝苍白核 54%、VLM 58%、孤束核 (NTS) 中部 39% 和尾 NTS 33% 中诱导 Fos 表达。脑室内注射 Nesfatin-1 显着减少胃排空。这些数据表明，NUCB2/nesfatin-1 免疫反应性分布在参与调节应激反应和由急性心理应激源激活的内脏功能的小鼠大脑区域，这表明 nesfatin-1 可能在应激反应的传出部分中发挥作用。
Nesfatin-1 reduces food intake when injected centrally in rodents. We recently described wide distribution of nucleobindin2 (NUCB2)/nesfatin-1 immunoreactivity in rat brain autonomic nuclei activated by various stressors. We used C57BL/6 mice to localize brain NUCB2/nesfatin-1 immunoreactivity and assessed activation of NUCB2/nesfatin 1 neurons after water avoidance stress (WAS). Gastric emptying of a non-nutrient liquid was also determined. NUCB2/nesfatin-1 immunoreactivity was detected in cortical areas including piriform, insular, cingulate and somatomotor cortices, the limbic system including amygdaloid nuclei, hippocampus and septum, the basal ganglia, bed nucleus of the stria terminalis, the thalamus including paraventricular and parafascicular nuclei, the hypothalamus including supraoptic, periventricular, paraventricular (PVN), arcuate nuclei and ventromedial and lateral hypothalamic areas. Intensely labeled NUCB2/nesfatin-1 neurons were detected in a previously undefined region which we named intermediate dorsomedial hypothalamus. In the brainstem, NUCB2/nesfatin-1 immunoreactivity was detected in the raphe nuclei, Edinger-Westphal nucleus, locus coeruleus (LC), lateral parabrachial nucleus, ventrolateral medulla (VLM) and dorsal vagal complex. WAS induced Fos expression in 35% of NUCB2/nesfatin-1-immunoreactive neurons in the PVN, 50% in the LC, 54% in the rostral raphe pallidus, 58% in the VLM, 39% in the middle part of the nucleus of the solitary tract (NTS) and 33% in the caudal NTS. Nesfatin-1 injected intracerebroventricularly significantly decreased gastric emptying. These data showed that NUCB2/nesfatin-1 immunoreactivity is distributed in mouse brain areas involved in the regulation of stress response and visceral functions activated by an acute psychological stressor suggesting that nesfatin-1 might play a role in the efferent component of the stress response.
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