Ischemic Stroke Disrupts Sleep Homeostasis in Middle-Aged Mice.
Ischemic Stroke Disrupts Sleep Homeostasis in Middle-Aged Mice.
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DOI：
10.3390/cells11182818
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发表时间：
2022-09-09
期刊：
影响因子：
6
通讯作者：
Thakkar, Mahesh M.
中科院分区：
文献类型：
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作者：
Sharma, Rishi;Chischolm, Abigail;Parikh, Meet;Qureshi, Adnan, I;Sahota, Pradeep;Thakkar, Mahesh M.
睡眠障碍，包括失眠和白天过度嗜睡，在缺血性卒中（IS）患者中非常普遍，严重影响恢复和康复工作。然而，IS如何导致睡眠障碍尚不清楚。在中年C57 BL/6 J小鼠上进行三个实验，用睡眠记录电极和/或进行1小时的大脑中动脉（MCAO;中风组）或假手术（Sham组）闭塞以诱导IS。再灌注48小时后，（a）实验1证实感觉运动缺陷（使用Garcia量表）和梗死（使用TTC染色）;（B）实验2检查IS对该小鼠模型中的质量的影响（睡眠潜伏期和NREM增量功率）和数量（c）实验3采用睡眠剥夺（SD）和恢复睡眠（RS）两种实验范式，观察IS对睡眠稳态的影响。中风小鼠表现出（a）感觉运动缺陷和脑梗死之间的显著相关性;（B）在光（非活动）期间的失眠样症状（增加的睡眠潜伏期、减少的NREM持续时间和Δ功率）和在暗（活动）期间的日间嗜睡样症状，模仿IS患者的睡眠;和（c）睡眠压力（在SD期间）和睡眠消散（在RS期间）的标志物的损伤。我们的研究结果表明，IS破坏睡眠稳态，导致睡眠障碍。
Sleep disturbances, including insomnia and excessive daytime sleepiness, are highly prevalent in patients with ischemic stroke (IS), which severely impacts recovery and rehabilitation efforts. However, how IS induces sleep disturbances is unclear. Three experiments were performed on middle-aged C57BL/6J mice, instrumented with sleep recording electrodes and/or subjected to 1 h of middle cerebral artery (MCAO; Stroke group) or sham (Sham group) occlusion to induce IS. After 48 h of reperfusion (a) experiment 1 verified sensorimotor deficit (using Garcia scale) and infarction (using TTC staining) in this mouse model; (b) experiment 2 examined the effects of IS on the quality (sleep latency and NREM delta power) and quantity (duration) of sleep; and (c) experiment 3 determined the effects of IS on sleep homeostasis using sleep deprivation (SD) and recovery sleep (RS) paradigm. Stroke mice display (a) a significant correlation between sensorimotor deficit and cerebral infarction; (b) insomnia-like symptoms (increased sleep latency, reduced NREM duration and delta power) during the light (inactive) period and daytime sleepiness-like symptoms during the dark (active) period mimicking sleep in IS patients; and (c) impairments in the markers of sleep pressure (during SD) and sleep dissipation (during RS). Our results suggest that IS disrupts sleep homeostasis to cause sleep disturbances.
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影响因子：
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