Indocyanine Green (ICG) Fluorescence Is Dependent on Monomer with Planar and Twisted Structures and Inhibited by H-Aggregation.
Indocyanine Green (ICG) Fluorescence Is Dependent on Monomer with Planar and Twisted Structures and Inhibited by H-Aggregation.
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DOI：
10.3390/ijms241713030
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发表时间：
2023-08-22
影响因子：
5.6
通讯作者：
Kundra, Vikas
中科院分区：
文献类型：
--
作者：
Chon, Bonghwan;Ghann, William;Uddin, Jamal;Anvari, Bahman;Kundra, Vikas
关键词：
吲哚青绿(ICG)作为近红外(NIR)荧光染料的光学性质取决于溶剂介质的性质和染料的浓度。在水的ICG吸收光谱中，高浓度时在700 nm处有最大吸收峰，表明存在H-聚集体。经ICG稀释后，主吸收峰位于780 nm处，为单体。而在乙醇中，ICG的最大吸收波长为780 nm，且不同浓度的ICG的吸收光谱形状相同，说明ICG在乙醇中只以单体形式存在，没有H-聚集体。我们发现，发射是由单体形式引起的，并随着H-聚集体的形成而减少。在荧光光谱中，低浓度时以820 nm发射带为主，高浓度时发射峰转换为880 nm，表明ICG的分子内扭曲电荷转移(TICT)过程是一种新的形式。ICG在乙醇和水中的近红外荧光强度在NIR-I区和-II区分别约为水中的12倍和9倍。我们提出了ICG的能量图来描述ICG通过单体、H-聚集体和TICT单体等结构的吸收和发射跃迁。
The optical properties of indocyanine green (ICG) as a near-infrared (NIR) fluorescence dye depend on the nature of the solvent medium and the dye concentration. In the ICG absorption spectra of water, at high concentrations, there were absorption maxima at 700 nm, implying H-aggregates. With ICG dilution, the main absorption peak was at 780 nm, implying monomers. However, in ethanol, the absorption maximum was 780 nm, and the shapes of the absorption spectra were identical regardless of the ICG concentration, indicating that ICG in ethanol exists only as a monomer without H-aggregates. We found that emission was due to the monomer form and decreased with H-aggregate formation. In the fluorescence spectra, the 820 nm emission band was dominant at low concentrations, whereas at high concentrations, we found that the emission peaks were converted to 880 nm, suggesting a new form via the twisted intramolecular charge transfer (TICT) process of ICG. The NIR fluorescence intensity of ICG in ethanol was approximately 12- and 9-times brighter than in water in the NIR-I and -II regions, respectively. We propose an energy diagram of ICG to describe absorptive and emissive transitions through the ICG structures such as the monomer, H-aggregated, and TICT monomer forms.
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影响因子：
4.1
作者：
Giraudeau, Camille;Moussaron, Albert;Frochot, Celine
通讯作者：
Frochot, Celine
DOI：
10.1021/acs.jpca.1c00629
发表时间：
2021-04-15
期刊：
The journal of physical chemistry. A
影响因子：
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作者：
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通讯作者：
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