Effect of intermittent low-frequency electrical stimulation on the rat gastrocnemius muscle.
Effect of intermittent low-frequency electrical stimulation on the rat gastrocnemius muscle.
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DOI：
10.1155/2013/480620
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发表时间：
2013
影响因子：
--
通讯作者：
Nakazato K
中科院分区：
文献类型：
--
作者：
Tsutaki A;Ogasawara R;Kobayashi K;Lee K;Kouzaki K;Nakazato K
低频神经肌肉电刺激（NMES）已被用作耐力运动模型。本研究旨在测试低频NMES是否会增加合成代谢信号分子的磷酸化并诱导骨骼肌肥大，正如高频NMES所见。采用Sprague-Dawley大鼠进行1次运动（运动后立即（0后）和3小时（3后）进行解剖）和6次训练。所有实验组均分为高、低频刺激（高频：100 Hz；低频：10 Hz）。采用周期性酸-希夫（PAS）染色研究II型纤维的活化，采用western blot分析（WB）检测NMES是否导致细胞内合成代谢信号传导。首先，我们研究了运动的急性效应。PAS染色显示I型和II型纤维均发生糖原消耗。WB结果显示，HFS显著提高了p70S6K磷酸化水平，但与LFS差异不显著。相比之下，LFS增加了ERK 1/2的磷酸化。在6次训练中，HFS和LFS均显著提高了肌肉的湿重和肌原纤维蛋白。综上所述，LFS对骨骼肌肥厚的合成代谢作用与HFS相似，但介导的信号通路可能不同。
Low-frequency neuromuscular electrical stimulation (NMES) has been used as an endurance exercise model. This study aimed to test whether low-frequency NMES increases the phosphorylation of anabolic signaling molecules and induces skeletal muscle hypertrophy, as seen with high-frequency NMES. Using Sprague-Dawley rats, 1 bout of exercise (with dissection done immediately (Post0) and 3 h (Post3) after exercise) and another 6 sessions of training were performed. All experimental groups consisted of high- and low-frequency stimulation (HFS: 100 Hz; LFS: 10 Hz). Periodic acid-Schiff (PAS) staining was conducted to investigate type II fiber activation, and western blot analysis (WB) was conducted to examine whether NMES leads to anabolic intracellular signaling. At first, we examined the acute effect of exercise. PAS staining revealed that glycogen depletion occurred in both type I and type II fibers. WB results demonstrated that p70S6K phosphorylation was significantly increased by HFS, but there was no significant difference with LFS. In contrast, ERK 1/2 phosphorylation was increased by LFS at Post0. In the 6-session training, the wet weight and myofibrillar protein were significantly increased by both HFS and LFS. In conclusion, LFS has a similar anabolic effect for skeletal muscle hypertrophy as HFS, but the mediating signaling pathway might differ.
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影响因子：
3.7
作者：
Bloemberg D;Quadrilatero J
通讯作者：
Quadrilatero J
影响因子：
3.3
作者：
Haddad, F;Qin, AX;Baldwin, KM
通讯作者：
Baldwin, KM
影响因子：
3.2
作者：
Mitchell, Bernhard;Weber, Juergen W.;Trupke, Thorsten
通讯作者：
Trupke, Thorsten
影响因子：
5.5
作者：
Aagaard, P;Andersen, JL;Simonsen, EB
通讯作者：
Simonsen, EB
影响因子：
5.5
作者：
Bolster, DR;Kubica, N;Jefferson, LS
通讯作者：
Jefferson, LS