Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis.
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DOI：
10.1371/journal.pone.0035273
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Quadrilatero J
中科院分区：
文献类型：
--
作者：
Bloemberg D;Quadrilatero J
骨骼肌是由具有不同形态、功能和代谢特性的纤维组成的异质组织。不同的肌肉含有不同比例的纤维类型；因此，准确的识别非常重要。许多组织化学方法被用来确定肌纤维类型；然而，这些技术有几个缺点。免疫荧光分析是一种灵敏的方法，允许在单个横截面上同时评估大量纤维上的多个MHC亚型，并提供了一种更精确的识别纤维类型的方法。在这项研究中，我们用多色免疫荧光分析方法对10只大鼠和10只小鼠骨骼肌以及人股外侧肌（VL）的纯纤维和杂交纤维类型分布进行了研究。此外，我们还测定了纤维类型特异性横截面积（CSA）、琥珀酸脱氢酶（SDH）活性和α-甘油磷酸脱氢酶（GPD）活性。使用这种方法，我们能够很容易地识别大鼠、小鼠和人类肌肉中的纯纤维和杂交纤维群体。杂交纤维在所有物种中都被发现，并且在一些大鼠和小鼠的肌肉中占了很大一部分。例如，大鼠混合腓肠肌（MG）含有12.2%的杂交纤维，而小鼠白胫前肌（WTA）含有12.1%的杂交纤维。总之，我们概述了一种简单而高效的方法来测定多种物种骨骼肌中MHC的表达。此外，我们还提供了大量大鼠和小鼠肌肉中纯纤维和杂交纤维类型分布、纤维CSA以及相对纤维类型特异性SDH和GPD活性的有用资源。
Skeletal muscle is a heterogeneous tissue comprised of fibers with different morphological, functional, and metabolic properties. Different muscles contain varying proportions of fiber types; therefore, accurate identification is important. A number of histochemical methods are used to determine muscle fiber type; however, these techniques have several disadvantages. Immunofluorescence analysis is a sensitive method that allows for simultaneous evaluation of multiple MHC isoforms on a large number of fibers on a single cross-section, and offers a more precise means of identifying fiber types. In this investigation we characterized pure and hybrid fiber type distribution in 10 rat and 10 mouse skeletal muscles, as well as human vastus lateralis (VL) using multicolor immunofluorescence analysis. In addition, we determined fiber type-specific cross-sectional area (CSA), succinate dehydrogenase (SDH) activity, and α-glycerophosphate dehydrogenase (GPD) activity. Using this procedure we were able to easily identify pure and hybrid fiber populations in rat, mouse, and human muscle. Hybrid fibers were identified in all species and made up a significant portion of the total population in some rat and mouse muscles. For example, rat mixed gastrocnemius (MG) contained 12.2% hybrid fibers whereas mouse white tibialis anterior (WTA) contained 12.1% hybrid fibers. Collectively, we outline a simple and time-efficient method for determining MHC expression in skeletal muscle of multiple species. In addition, we provide a useful resource of the pure and hybrid fiber type distribution, fiber CSA, and relative fiber type-specific SDH and GPD activity in a number of rat and mouse muscles.
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影响因子：
3.4
作者：
Landisch, Rachel M.;Kosir, Allison M.;Nelson, Steven A.;Baltgalvis, Kristen A.;Lowe, Dawn A.
通讯作者：
Lowe, Dawn A.
DOI：
10.1006/bbrc.2000.2768
发表时间：
2000-05-27
影响因子：
3.1
作者：
Lucas, CA;Kang, LHD;Hoh, JFY
通讯作者：
Hoh, JFY
影响因子：
3.3
作者：
Harrison, BC;Allen, DL;Leinwand, LA
通讯作者：
Leinwand, LA
DOI：
10.1152/ajpcell.2001.280.3.c637
发表时间：
2001-03-01
影响因子：
5.5
作者：
Allen, DL;Harrison, BC;Leinwand, LA
通讯作者：
Leinwand, LA
影响因子：
3.3
作者：
Andersen, JL;Gruschy-Knudsen, T;Schiaffino, S
通讯作者：
Schiaffino, S