Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica.

Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica.
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DOI:
10.1016/j.mimet.2015.03.010
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发表时间:
2015-05
影响因子:
2.2
通讯作者:
Qi, Fengxia
Qi, Fengxia
中科院分区:
生物学4区
文献类型:
--
作者:
Zhou, Peng;Li, Xiaoli;Qi, Fengxia

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以备选西格玛因子ecf3为靶点,成功建立了首个绒毛菌属的无标记诱变系统。该系统将为微孔菌多基因诱变、基因功能分析和基因调控研究提供有价值的工具。
Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella.
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