Interactions between the Prophage 919TP and Its Vibrio cholerae Host: Implications of gmd Mutation for Phage Resistance, Cell Auto-Aggregation, and Motility.
Interactions between the Prophage 919TP and Its Vibrio cholerae Host: Implications of gmd Mutation for Phage Resistance, Cell Auto-Aggregation, and Motility.
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前噬菌体919TP和其霍乱弧菌宿主之间的相互作用：gmd突变对噬菌体抗性、细胞自动聚集和运动性的影响。
DOI：
10.3390/v13122342
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发表时间：
2021-11-23
期刊：
影响因子：
--
通讯作者：
Tan D
中科院分区：
文献类型：
--
作者：
Li N;Zeng Y;Hu B;Zhu T;Svenningsen SL;Middelboe M;Tan D
原噬菌体919 TP广泛分布于霍乱弧菌中，并被诱导产生游离的φ 919 TP噬菌体颗粒。然而，原噬菌体φ 919 TP，诱导的噬菌体颗粒，与其宿主之间的相互作用仍然未知。特别是，噬菌体抗性机制和潜在的健身权衡，导致噬菌体抗性，尚未解决。在这项研究中，我们研究了一个前噬菌体919 TP缺失的变异体的霍乱弧菌和它的相互作用的诱导前噬菌体（φ 919 TP cI-）的修改裂解变体。具体地，通过用裂解性噬菌体φ 919 TP cI-攻击原噬菌体缺失的变体来分离噬菌体抗性突变体。进一步对野生型和φ 919 TP cI-抗性突变体的比较基因组分析预测，噬菌体φ 919 TP cI-选择在脂多糖（LPS）O-抗原生物合成的关键步骤中含有突变的噬菌体抗性突变体，导致gmd基因中的单碱基对缺失。我们的研究表明，gmd介导的O抗原缺陷可导致多效性表型，例如，细胞自动聚集和减少的群集运动，强调噬菌体驱动的多样化在霍乱弧菌中的作用。所开发的方法有助于识别宿主特异性的遗传决定因素，并用于探索噬菌体-宿主相互作用的分子机制。我们的研究结果有助于理解原噬菌体促进的水平基因转移，并强调开发新的策略，以优化在细菌病原体控制中使用的peptide的潜力。
Prophage 919TP is widely distributed among Vibrio cholera and is induced to produce free φ919TP phage particles. However, the interactions between prophage φ919TP, the induced phage particle, and its host remain unknown. In particular, phage resistance mechanisms and potential fitness trade-offs, resulting from phage resistance, are unresolved. In this study, we examined a prophage 919TP-deleted variant of V. cholerae and its interaction with a modified lytic variant of the induced prophage (φ919TP cI-). Specifically, the phage-resistant mutant was isolated by challenging a prophage-deleted variant with lytic phage φ919TP cI-. Further, the comparative genomic analysis of wild-type and φ919TP cI--resistant mutant predicted that phage φ919TP cI- selects for phage-resistant mutants harboring a mutation in key steps of lipopolysaccharide (LPS) O-antigen biosynthesis, causing a single-base-pair deletion in gene gmd. Our study showed that the gmd-mediated O-antigen defect can cause pleiotropic phenotypes, e.g., cell autoaggregation and reduced swarming motility, emphasizing the role of phage-driven diversification in V. cholerae. The developed approach assists in the identification of genetic determinants of host specificity and is used to explore the molecular mechanism underlying phage-host interactions. Our findings contribute to the understanding of prophage-facilitated horizontal gene transfer and emphasize the potential for developing new strategies to optimize the use of phages in bacterial pathogen control.
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影响因子：
2.1
作者：
INOUE, T;MATSUZAKI, S;TANAKA, S
通讯作者：
TANAKA, S
影响因子：
3.7
作者：
Salmon, KA;Freedman, O;DuBow, MS
通讯作者：
DuBow, MS
影响因子：
5.4
作者：
HELLER, K;BRAUN, V
通讯作者：
BRAUN, V
影响因子：
82.9
作者：
Dedrick, Rebekah M.;Guerrero-Bustamante, Carlos A.;Spencer, Helen
通讯作者：
Spencer, Helen
影响因子：
3.2
作者：
Nesper, J;Kapfhammer, D;Reidl, J
通讯作者：
Reidl, J