Spatial Memory Engram in the Mouse Retrosplenial Cortex.
Spatial Memory Engram in the Mouse Retrosplenial Cortex.
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DOI：
10.1016/j.cub.2018.05.002
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发表时间：
2018-06-18
期刊：
影响因子：
--
通讯作者：
Sengpiel F
中科院分区：
文献类型：
--
作者：
Milczarek MM;Vann SD;Sengpiel F
记忆依赖于神经元特性的持久适应，这些特性是由刺激驱动的可塑性变化引起的。突触的加强(和减弱)导致了功能集合的建立。假设这样的集合(或印记)在记忆获取期间被激活，并在记忆检索时被重新激活。脾后皮质(RSC)是支持记忆的关键脑区，包括人类的情景记忆和地形图记忆，以及啮齿动物的空间记忆。异粒状RSC与背流视区紧密相连，并含有类地点细胞和头部细胞，使其成为整合导航信息的首选。虽然以前的报告描述了导航任务中RSC集合的招募，但这种集合从未被跟踪足够长的时间来提供稳定印记的证据，也没有与长期记忆的保持有关。在这里，我们使用活体双光子成像来分析RSC内6,000多个神经元的活动模式。八只小鼠接受了空间记忆任务的训练。伴随着学习的是在3周的时间里逐渐出现的特定于背景的神经元活动模式，这种模式在3周多后的提取时恢复。记忆印记的稳定性预示着遗忘的程度；记忆印记越稳定，表现越好。这为空间记忆巩固和RSC印记形成之间的相互依赖提供了直接证据。我们的结果表明，RSC在神经元集合水平上参与了空间记忆存储。纵向C-fos成像显示，小鼠脾后空间记忆印记随着学习的进行逐渐变得更加稳定，并在数周内保持，记忆保持的程度与印记Milczarek等人的稳定性有关。在活体C-FOS成像中识别小鼠脾后皮质的空间记忆印记。随着学习，印记逐渐变得更稳定，并在几周内保持不变。记忆保持的程度与记忆的稳定性有关，突出了记忆巩固的可能机制。
Memory relies on lasting adaptations of neuronal properties elicited by stimulus-driven plastic changes. The strengthening (and weakening) of synapses results in the establishment of functional ensembles. It is presumed that such ensembles (or engrams) are activated during memory acquisition and re-activated upon memory retrieval. The retrosplenial cortex (RSC) has emerged as a key brain area supporting memory, including episodic and topographical memory in humans, as well as spatial memory in rodents. Dysgranular RSC is densely connected with dorsal stream visual areas and contains place-like and head-direction cells, making it a prime candidate for integrating navigational information. While previous reports describe the recruitment of RSC ensembles during navigational tasks, such ensembles have never been tracked long enough to provide evidence of stable engrams and have not been related to the retention of long-term memory. Here, we used in vivo 2-photon imaging to analyze patterns of activity of over 6,000 neurons within dysgranular RSC. Eight mice were trained on a spatial memory task. Learning was accompanied by the gradual emergence of a context-specific pattern of neuronal activity over a 3-week period, which was re-instated upon retrieval more than 3 weeks later. The stability of this memory engram was predictive of the degree of forgetting; more stable engrams were associated with better performance. This provides direct evidence for the interdependence of spatial memory consolidation and RSC engram formation. Our results demonstrate the participation of RSC in spatial memory storage at the level of neuronal ensembles. Longitudinal C-fos imaging reveals retrosplenial spatial memory engrams in mice Engrams become progressively more stable with learning and are maintained over weeks The degree of memory retention is related to the stability of the engrams Milczarek et al. identify spatial memory engrams in the mouse retrosplenial cortex using in vivo C-fos imaging. The engrams become progressively more stable with learning and are maintained over several weeks. The degree of memory retention is related to the stability of the engrams, highlighting a possible mechanism for memory consolidation.
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10.1098/rstb.2016.0159
发表时间：
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期刊：
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影响因子：
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