In vivo assessment of the placental anatomy and perfusion in a mouse model of intrauterine inflammation.
In vivo assessment of the placental anatomy and perfusion in a mouse model of intrauterine inflammation.
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DOI：
10.1002/jmri.25867
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Burd I
中科院分区：
文献类型：
--
作者：
Wu D;Lei J;Jia B;Xie H;Zhu Y;Xu J;Mori S;Zhang J;Burd I
MRI为检查胎盘功能提供了有用的标记物。宫内炎症引起的胎盘损伤的MRI特征--怀孕期间的一种常见风险--并不为人所知。我们的目的是研究结构MRI和体素内非相干运动(IVIM)成像在检查宫内炎症小鼠急性胎盘损伤以及妊娠相关性胎盘变化中的能力。前瞻性研究。妊娠CD1小鼠在胚胎第15天(E15，n=40个来自6个母鼠的胎盘)和E17天进行扫描。E17时，用脂多糖(脂多糖，3只母鼠胎盘，n=25只)或假损伤(3只母鼠胎盘25只)造成宫内损伤。在11.7Tesla Bruker扫描仪上，使用快速自旋回波序列和扩散加权回波平面成像(EPI)序列进行活体MRI检查。用T2加权磁共振成像评估胎盘体积。取S/mm~2范围内的15个b值，在受限视野内进行IVIM成像，采用两步拟合法计算拟扩散分数(F)、拟扩散系数(D*)和组织水系数(D)。采用双因素方差分析评价两组间的差异。胎盘体积从E15到E17增加了约21%(p<；0.01)，并在暴露后6小时观察到15%的体积减少(p<；0.01)。E15和E17假手术组的IVIM参数(f、D*和f·D*)相似，但与假手术组(P<；0.001)相比，脂多糖暴露组胎盘的IVIM参数显著降低(P<；0.05)。D值从E15降至E17(p<；0.05)，内毒素暴露后D值进一步降低(p<；0.05)。在组织学上，内毒素暴露组的胎盘面积和血管密度减少，并伴随着妊娠依赖性的改变。我们的结果提示结构MRI和IVIM测量是检测宫内炎症后急性胎盘损伤的潜在标志物。
MRI provides useful markers to examine placental function. MRI features of placental injury due to intrauterine inflammation—a common risk during pregnancy, are not well-known. We aim to investigate the capability of structural MRI and intra-voxel incoherent motion (IVIM) imaging in examining acute placental injury in a mouse model of intrauterine inflammation, as well as gestation-dependent placental changes. Prospective study. Pregnant CD1 mice were scanned on embryonic day 15 (E15, n=40 placentas from six dams) and E17. On E17, mice were subjected to intrauterine injury by exposure to lipopolysaccharide (LPS, n=25 placentas from three dams) or sham injury (n=25 placentas from three dams). In vivo MRI was performed on an 11.7 Tesla Bruker scanner, using a fast spin-echo sequence and a diffusion-weighted echo-planar imaging (EPI) sequence. T2-weighted MRI was acquired to evaluate placental volume. IVIM imaging was performed in a restricted field-of-view using 15 b-values from 10–800 s/mm2, based on which, the pseudo-diffusion fraction (f), pseudo-diffusion coefficient (D*), and tissue water coefficient (D) were estimated with a two-step fitting procedure. Two-way ANOVA was used to evaluate the group differences. The placental volume increased by approximately 21% from E15 to E17 (p<0.01), and a 15% volume loss was observed at 6hrs after LPS exposure (p<0.01). IVIM parameters (f, D*, and f·D*) were similar between the E15 and E17 sham groups (p>0.05), which significantly reduced in the LPS-exposed placentas compared to the shams (p<0.001). D values decreased from E15 to E17 (p<0.05), which were further reduced after LPS exposure (p<0.05). Decreased placental area and vascular density were histologically identified in the LPS-exposed group, along with gestation-dependent changes. Our results suggested structural MRI and IVIM measurements are potential markers for detecting acute placental injury after intrauterine inflammation.
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影响因子：
3
作者：
Gowland, P
通讯作者：
Gowland, P
影响因子：
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作者：
Andescavage NN;du Plessis A;Limperopoulos C
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