Using dynamic contrast-enhanced MRI to quantitatively characterize maternal vascular organization in the primate placenta.
Using dynamic contrast-enhanced MRI to quantitatively characterize maternal vascular organization in the primate placenta.
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DOI：
10.1002/mrm.25264
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发表时间：
2015-04
影响因子：
3.3
通讯作者：
Kroenke, Christopher D.
中科院分区：
文献类型：
--
作者：
Frias, Antonio E.;Schabel, Matthias C.;Roberts, Victoria H. J.;Tudorica, Alina;Grigsby, Peta L.;Oh, Karen Y.;Kroenke, Christopher D.
灵长类胎盘的母体微血管系统被组织成10-20个灌注域，这些灌注域在功能上被优化以促进营养交换以支持胎儿生长。本研究描述了一种动态对比增强磁共振成像（DCE-MRI）方法，用于识别血管域，并量化其中的母体血流。恒河猴在怀孕第133天（G133，期限=165天）进行多普勒超声（US）程序，DCE-MRI和剖腹产分娩。通过静脉注射造影剂（CR）团注获得的系列T1加权图像进行分析，以获得胎盘的CR到达时间图。到达时间图的分水岭分割确定了16个灌注域。这些域的数量和位置对应于交付后观察到的解剖学子叶单位。通过每个灌注域的CR波前分析能够确定体积流量，其范围为9.03至44.9 mL/sec（25.2 ± 10.3 mL/sec）。这些估计得到多普勒超声结果的支持。这里描述的DCE-MRI分析提供了灵长类动物胎盘中母体灌注域数量的定量估计，并估计每个域内的流量。预期这项技术的扩展是研究胎盘功能的非人灵长类动物模型的产科并发症。
The maternal microvasculature of the primate placenta is organized into 10-20 perfusion domains that are functionally optimized to facilitate nutrient exchange to support fetal growth. This study describes a dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) method for identifying vascular domains, and quantifying maternal blood flow in them. A rhesus macaque on the 133rd day of pregnancy (G133, term=165 days) underwent Doppler ultrasound (US) procedures, DCE-MRI, and Cesarean-section delivery. Serial T1-weighted images acquired throughout intravenous injection of a contrast reagent (CR) bolus were analyzed to obtain CR arrival time maps of the placenta. Watershed segmentation of the arrival time map identified 16 perfusion domains. The number and location of these domains corresponded to anatomical cotyledonary units observed following delivery. Analysis of the CR wave front through each perfusion domain enabled determination of volumetric flow, which ranged from 9.03 to 44.9 mL/sec (25.2 ± 10.3 mL/sec). These estimates are supported by Doppler US results. The DCE-MRI analysis described here provides quantitative estimates of the number of maternal perfusion domains in a primate placenta, and estimates flow within each domain. Anticipated extensions of this technique are to the study placental function in nonhuman primate models of obstetric complications.
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影响因子：
1.3
作者：
Podrasky, Ann E.;Javitt, Marcia C.;Zelop, Carolyn M.
通讯作者：
Zelop, Carolyn M.
影响因子：
15.9
作者：
POWER, GG;LONGO, LD;FROSTER, RE
通讯作者：
FROSTER, RE
影响因子：
3
作者：
Chalouhi, G. E.;Deloison, B.;Salomon, L. J.
通讯作者：
Salomon, L. J.
影响因子：
3.8
作者：
Arthuis, C. J.;Novell, A.;Perrotin, F.
通讯作者：
Perrotin, F.
影响因子：
--
作者：
BENIRSCHKE, K
通讯作者：
BENIRSCHKE, K