A CLASP-modulated cell edge barrier mechanism drives cell-wide cortical microtubule organization in Arabidopsis.
A CLASP-modulated cell edge barrier mechanism drives cell-wide cortical microtubule organization in Arabidopsis.
复制标题
DOI：
10.1038/ncomms1444
复制
发表时间：
2011-08-16
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
众所周知，植物细胞皮层中微管的平行顺序决定了细胞扩张的方向，但仍不清楚微管的方向是如何控制的，特别是在细胞范围内。在这里，我们通过4D成像和计算建模表明，植物细胞多面体几何形状提供了空间输入，确定阵列方向和异质性。当遇到尖锐的细胞边缘时，微管会脱落，而那些平行于这些尖锐边缘的微管则会保留下来。然而，边缘诱导的微管解聚被微管相关蛋白CLASP克服，该蛋白在特定的细胞边缘积累，使微管能够在尖锐边缘周围生长，并促进跨越相邻细胞表面的微管束的形成。通过计算建模动态的“微管在立方体上”与边缘差异允许微管通过，我们表明，CLASP边缘复杂的是一个“可调”微管组织者，与固有的灵活性，以产生许多皮质阵列模式中观察到的性质。微管在植物细胞中是如何正确组织的还不清楚。Ambrose等人使用4D成像和计算机建模来显示尖锐的细胞边缘诱导微管解聚，并且微管相关蛋白CLASP减轻了这一过程以调节阵列组织。
It is well known that the parallel order of microtubules in the plant cell cortex defines the direction of cell expansion, yet it remains unclear how microtubule orientation is controlled, especially on a cell-wide basis. Here we show through 4D imaging and computational modelling that plant cell polyhedral geometry provides spatial input that determines array orientation and heterogeneity. Microtubules depolymerize when encountering sharp cell edges head-on, whereas those oriented parallel to those sharp edges remain. Edge-induced microtubule depolymerization, however, is overcome by the microtubule-associated protein CLASP, which accumulates at specific cell edges, enables microtubule growth around sharp edges and promotes formation of microtubule bundles that span adjacent cell faces. By computationally modelling dynamic 'microtubules on a cube' with edges differentially permissive to microtubule passage, we show that the CLASP-edge complex is a 'tuneable' microtubule organizer, with the inherent flexibility to generate the numerous cortical array patterns observed in nature. How microtubules are organized correctly in plant cells is not well understood. Ambrose et al. use 4D imaging and computer modelling to show that sharp cell edges induce microtubule depolymerization and that the microtubule-associated protein CLASP mitigates this process to modulate array organization.
登录
查看更多内容
DOI：
10.1083/jcb.200301147
发表时间：
2003-06-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Janson ME;de Dood ME;Dogterom M
通讯作者：
Dogterom M
影响因子：
11.6
作者：
Dixit, R;Cyr, R
通讯作者：
Cyr, R
影响因子：
11.8
作者：
Al-Bassam, Jawdat;Kim, Hwajin;Brouhard, Gary;van Oijen, Antoine;Harrison, Stephen C.;Chang, Fred
通讯作者：
Chang, Fred
影响因子：
7.5
作者：
Ehrhardt, David W.
通讯作者：
Ehrhardt, David W.
影响因子：
8.6
作者：
DOGTEROM, M;LEIBLER, S
通讯作者：
LEIBLER, S