The biological applications of DNA nanomaterials: current challenges and future directions.
The biological applications of DNA nanomaterials: current challenges and future directions.
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DOI：
10.1038/s41392-021-00727-9
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发表时间：
2021-10-08
影响因子：
39.3
通讯作者：
Lin Y
中科院分区：
文献类型：
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作者：
Ma W;Zhan Y;Zhang Y;Mao C;Xie X;Lin Y
DNA是一种遗传物质，在过去的几十年里，DNA纳米技术已经在不同的科学方向上用于各种生物学应用，包括组织再生、疾病预防、炎症抑制、生物成像、生物传感、诊断、抗肿瘤药物递送和治疗。随着DNA纳米技术的迅速发展，基于经典的Watson-Crick碱基配对的分子自组装，设计出了大量不同形状和大小的DNA纳米材料。一些DNA材料可以在功能上改变细胞的生物学行为，如细胞迁移、细胞增殖、细胞分化、自噬和抗炎作用等。一些单链DNA（ssDNA）或RNA通过自身配对形成二级结构，称为适体（aptamer），具有靶向作用，通过指数富集配体系统进化（SELEX）技术筛选，用于肿瘤的靶向诊断和治疗。一些具有三维纳米结构和稳定结构的DNA纳米材料被研究作为药物载体系统，用于递送多种抗肿瘤药物或基因治疗药物。虽然功能性DNA纳米结构以其创新的设计和制备策略推动了DNA纳米技术的发展，并在生物和医学应用中显示出巨大的潜力，但DNA材料最终应用于真实的生活中还有很长的路要走。本文综述了各种DNA纳米材料的结构发展历史，介绍了不同DNA纳米材料的原理，总结了它们在不同领域的生物学应用，并讨论了当前的挑战和未来的发展方向。
DNA, a genetic material, has been employed in different scientific directions for various biological applications as driven by DNA nanotechnology in the past decades, including tissue regeneration, disease prevention, inflammation inhibition, bioimaging, biosensing, diagnosis, antitumor drug delivery, and therapeutics. With the rapid progress in DNA nanotechnology, multitudinous DNA nanomaterials have been designed with different shape and size based on the classic Watson–Crick base-pairing for molecular self-assembly. Some DNA materials could functionally change cell biological behaviors, such as cell migration, cell proliferation, cell differentiation, autophagy, and anti-inflammatory effects. Some single-stranded DNAs (ssDNAs) or RNAs with secondary structures via self-pairing, named aptamer, possess the ability of targeting, which are selected by systematic evolution of ligands by exponential enrichment (SELEX) and applied for tumor targeted diagnosis and treatment. Some DNA nanomaterials with three-dimensional (3D) nanostructures and stable structures are investigated as drug carrier systems to delivery multiple antitumor medicine or gene therapeutic agents. While the functional DNA nanostructures have promoted the development of the DNA nanotechnology with innovative designs and preparation strategies, and also proved with great potential in the biological and medical use, there is still a long way to go for the eventual application of DNA materials in real life. Here in this review, we conducted a comprehensive survey of the structural development history of various DNA nanomaterials, introduced the principles of different DNA nanomaterials, summarized their biological applications in different fields, and discussed the current challenges and further directions that could help to achieve their applications in the future.
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DOI：
10.3390/ma11071116
发表时间：
2018-06-29
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Beldjilali-Labro M;Garcia Garcia A;Farhat F;Bedoui F;Grosset JF;Dufresne M;Legallais C
通讯作者：
Legallais C
影响因子：
14
作者：
Boylan, Nicholas J.;Kim, Anthony J.;Suk, Jung Soo;Adstamongkonkul, Pichet;Simons, Brian W.;Lai, Samuel K.;Cooper, Mark J.;Hanes, Justin
通讯作者：
Hanes, Justin
DOI：
10.1080/07391102.1988.10507714
发表时间：
1988-10-01
影响因子：
4.4
作者：
BASU, HS;FEUERSTEIN, BG;MARTON, LJ
通讯作者：
MARTON, LJ
影响因子：
4.9
作者：
Atluri K;Seabold D;Hong L;Elangovan S;Salem AK
通讯作者：
Salem AK
影响因子：
82.9
作者：
通讯作者：
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