The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms.
The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms.
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DOI：
10.1371/journal.pone.0056017
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ebisu S
中科院分区：
文献类型：
--
作者：
Yamamoto R;Noiri Y;Yamaguchi M;Asahi Y;Maezono H;Kuboniwa M;Hayashi M;Ebisu S
生物被膜形成细胞不同于已充分表征的增殖细胞，并且在细胞外基质中聚集，即所谓的细胞外聚合物（EPS）。枯草芽孢杆菌的sinR基因编码已知参与生物膜中EPS的生物合成的转录调节因子。牙龈卟啉单胞菌（Porphyromonasgingivalis）是一种存在于牙龈下和根外的生物膜中的细菌，是引起进行性边缘性和难治性根尖周炎的主要病原菌之一。此外，牙龈卟啉单胞菌具有PGN_0088，其编码B的推定直系同源物。subtilis sinR.在此，我们研究了PGN_0088（sinR）对生物膜形成的作用。牙龈卟啉单胞菌菌株在磷酸盐缓冲盐水中在唾液涂覆的玻璃表面上形成生物膜。定量分析表明sinR无效突变体的生物膜由致密的胞外多糖组成。显微镜观察表明，由sinR突变体产生的胞外多糖水平的增加改变了EPS的形态，使其成为网状结构。此外，物理分析表明，胞外多糖在EPS中的富集增强了生物膜对流体动力学剪切力的抵抗力。本文呈现的结果表明，sinR在牙龈卟啉单胞菌菌株ATCC 33277作为胞外多糖积累的负介质的能力中起重要作用，并且与EPS的结构及其粘附于表面的力间接相关。
Biofilm-forming cells are distinct from well characterized planktonic cells and aggregate in the extracellular matrix, the so-called extracellular polymeric substances (EPS). The sinR gene of Bacillus subtilis encodes a transcriptional regulator that is known to be involved in the biosynthesis of EPS in biofilms. Porphyromonas gingivalis inhabits the subgingival and extraradicular biofilm of humans and is one of the primary pathogens that cause progressive marginal and refractory apical periodontitis. Furthermore, P. gingivalis possesses PGN_0088, which encodes a putative ortholog of B. subtilis sinR. Here, we investigated the role of PGN_0088 (sinR) on biofilm formation. P. gingivalis strains formed biofilms on saliva-coated glass surfaces in phosphate buffered saline. Quantitative analysis indicated that the biofilm of the sinR null mutant consisted of dense exopolysaccharide. Microscopic observations showed that the increased levels of exopolysaccharide produced by the sinR mutant changed the morphology of the EPS to a mesh-liked structure. Furthermore, physical analyses suggested that the enrichment of exopolysaccharide in the EPS enhanced the resistance of the biofilm to hydrodynamic shear force. The results presented here demonstrate sinR plays important roles in the ability of P. gingivalis strain ATCC 33277 to act as a negative mediator of exopolysaccharide accumulation and is indirectly associated with the structure of the EPS and the force of its adhesion to surfaces.
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影响因子：
3
作者：
COSTERTON, JW;GEESEY, GG;CHENG, KJ
通讯作者：
CHENG, KJ
影响因子：
3.6
作者：
Kearns, DB;Chu, F;Losick, R
通讯作者：
Losick, R
影响因子：
4.3
作者：
Genco, RJ
通讯作者：
Genco, RJ
EDNA释放和降解的调节会影响金黄色葡萄球菌生物膜成熟。
影响因子：
3.7
作者：
Mann EE;Rice KC;Boles BR;Endres JL;Ranjit D;Chandramohan L;Tsang LH;Smeltzer MS;Horswill AR;Bayles KW
通讯作者：
Bayles KW
影响因子：
4.2
作者：
Kuboniwa M;Amano A;Hashino E;Yamamoto Y;Inaba H;Hamada N;Nakayama K;Tribble GD;Lamont RJ;Shizukuishi S
通讯作者：
Shizukuishi S