Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis.
Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis.
复制标题
DOI：
10.1186/1471-2180-9-105
复制
发表时间：
2009-05-26
期刊：
影响因子：
4.2
通讯作者：
Shizukuishi S
中科院分区：
文献类型：
--
作者：
Kuboniwa M;Amano A;Hashino E;Yamamoto Y;Inaba H;Hamada N;Nakayama K;Tribble GD;Lamont RJ;Shizukuishi S
牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周病病原体，表达多种毒力因子，包括长（FimA）和短（Mfa）菌毛以及由精氨酸特异性（Rgp）和赖氨酸特异性（Kgp）半胱氨酸蛋白酶组成的牙龈卟啉蛋白酶。本研究的目的是检查这些组分在牙龈卟啉单胞菌的同型生物膜发育中的作用，以及在生物膜中胞外多糖的积累中的作用。在PBS或稀释的胰蛋白胨大豆肉汤（dTSB）中，在唾液涂覆的玻璃表面上形成生物膜。显微镜观察显示野生型菌株在PBS和dTSB中均形成具有致密基底单层和分散小菌落的生物膜。FimA缺陷型突变体在PBS中形成斑片状和小的微菌落，但生物体增殖并在dTSB中形成具有致密胞外多糖的粘性生物膜。Mfa突变体在PBS和dTSB中形成了高大的小菌落。在这两种条件下，Kgp突变体形成了明显厚的生物膜，充满了大的丛生菌落。RgpA/B双突变体在PBS中形成具有纤维状和高的小菌落的通道样生物膜。当在dTSB中研究该突变体时，峰的数量增加，并且形态改变为更高和松散包装的生物膜。此外，FimA的缺失降低了自聚集效率，而自聚集在Kgp和Mfa突变体中显著增加，与非增殖条件下生物膜结构的改变有明确的关联。与此相反，这种关联没有观察到的Rgp无效突变体。这些结果表明，FimA菌毛促进初始生物膜的形成，但对生物膜的成熟施加抑制调节，而Mfa和Kgp在生物膜的发育过程中具有抑制和调节作用。Rgp控制微菌落形态和生物量。总的来说，这些分子似乎协同作用以调节成熟牙龈卟啉单胞菌生物膜的发育。
Porphyromonas gingivalis, a periodontal pathogen, expresses a number of virulence factors, including long (FimA) and short (Mfa) fimbriae as well as gingipains comprised of arginine-specific (Rgp) and lysine-specific (Kgp) cysteine proteinases. The aim of this study was to examine the roles of these components in homotypic biofilm development by P. gingivalis, as well as in accumulation of exopolysaccharide in biofilms. Biofilms were formed on saliva-coated glass surfaces in PBS or diluted trypticase soy broth (dTSB). Microscopic observation showed that the wild type strain formed biofilms with a dense basal monolayer and dispersed microcolonies in both PBS and dTSB. A FimA deficient mutant formed patchy and small microcolonies in PBS, but the organisms proliferated and formed a cohesive biofilm with dense exopolysaccharides in dTSB. A Mfa mutant developed tall and large microcolonies in PBS as well as dTSB. A Kgp mutant formed markedly thick biofilms filled with large clumped colonies under both conditions. A RgpA/B double mutant developed channel-like biofilms with fibrillar and tall microcolonies in PBS. When this mutant was studied in dTSB, there was an increase in the number of peaks and the morphology changed to taller and loosely packed biofilms. In addition, deletion of FimA reduced the autoaggregation efficiency, whereas autoaggregation was significantly increased in the Kgp and Mfa mutants, with a clear association with alteration of biofilm structures under the non-proliferation condition. In contrast, this association was not observed in the Rgp-null mutants. These results suggested that the FimA fimbriae promote initial biofilm formation but exert a restraining regulation on biofilm maturation, whereas Mfa and Kgp have suppressive and regulatory roles during biofilm development. Rgp controlled microcolony morphology and biovolume. Collectively, these molecules seem to act coordinately to regulate the development of mature P. gingivalis biofilms.
登录
查看更多内容
影响因子：
3.5
作者：
Kuramitsu, H;Tokuda, M;Cho, MI
通讯作者：
Cho, MI
影响因子：
5.2
作者：
Labrecque, Julie;Bodet, Charles;Grenier, Daniel
通讯作者：
Grenier, Daniel
影响因子：
3.2
作者：
Davey, Mary E.;Duncan, Margaret J.
通讯作者：
Duncan, Margaret J.
DOI：
10.2741/2363
发表时间：
2007-05-01
期刊：
FRONTIERS IN BIOSCIENCE
影响因子：
--
作者：
Amano, Atsuo
通讯作者：
Amano, Atsuo
影响因子：
3.1
作者：
HAMADA, N;WATANABE, K;UMEMOTO, T
通讯作者：
UMEMOTO, T