Anti-inflammatory effect of (-)-epigallocatechin-3-gallate on Porphyromonas gingivalis lipopolysaccharide-stimulated fibroblasts and stem cells derived from human periodontal ligament.
Anti-inflammatory effect of (-)-epigallocatechin-3-gallate on Porphyromonas gingivalis lipopolysaccharide-stimulated fibroblasts and stem cells derived from human periodontal ligament.
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（ - ） - 表瓜蛋白3-gallate对卟啉单胞菌脂肪含糖刺激的成纤维细胞和干细胞源自人牙周韧带的抗炎作用。
DOI：
10.5051/jpis.2012.42.6.185
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发表时间：
2012-12
影响因子：
1.9
通讯作者：
Choi SH
中科院分区：
文献类型：
--
作者：
Jung IH;Lee DE;Yun JH;Cho AR;Kim CS;You YJ;Kim SJ;Choi SH
（-）-表没食子儿茶素-3-没食子酸酯（EGCG）已被报道在牙周炎中发挥抗炎和抗菌作用。然而，其确切的作用机制尚未确定。体外研究了EGCG对牙龈卟啉单胞菌细菌脂多糖（LPS）对人牙周韧带成纤维细胞（hPDLFs）和人牙周韧带干细胞（hPDLSCs）的抗炎作用。从健康年轻人中提取hPDLFs和hPDLSCs，并用EGCG和/或牙龈卟啉卟啉脂多糖处理。治疗1、3、5和7天后，分别采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯四唑溴化试验和溴脱氧尿苷试验评估细胞毒性和增殖作用。然后，采用实时聚合酶链反应（real- real polymerase chain reaction， PCR）技术，分别在治疗后0、6、24、48 h观察hPDLFs和hPDLSCs中白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α、骨保护素（OPG）、核因子κ b配体受体激活物（RANKL）和RANKL/OPG的基因表达。hPDLFs组和hPDLSCs组分别进行实验；1)不治疗，2)单独使用EGCG， 3)单独使用牙龈卟啉脂多糖，4)EGCG+P。gingivalis有限合伙人。EGCG浓度为20µM， P. gingivalis LPS浓度为20µg/mL时细胞毒作用最低，可作为进一步实验的浓度。在所有组中，hPDLFs和hPDLSCs的增殖均增加，但“单独EGCG”的增殖较少。real-time PCR结果显示，“单独处理”的hPDLFs和hPDLSCs与“未处理”细胞的基因表达相似。除hPDLSCs中的RANKL/OPG外，hPDLFs和hPDLSCs中的IL-1β、IL-6、TNF-α、RANKL和RANKL/OPG的基因表达在6 h时均显著升高。而在EGCG+P中，这些增加的基因表达则下调。EGCG对牙龈炎LPS的治疗效果较好。我们的研究结果表明，EGCG可以在hPDLFs和hPDLSCs中发挥抗炎作用，对抗牙周炎的主要病原体牙龈卟啉卟啉脂多糖。
(-)-epigallocatechin-3-gallate (EGCG) has been reported to exert anti-inflammatory and antibacterial effects in periodontitis. However, its exact mechanism of action has yet to be determined. The present in vitro study evaluated the anti-inflammatory effects of EGCG on human periodontal ligament fibroblasts (hPDLFs) and human periodontal ligament stem cells (hPDLSCs) affected by bacterial lipopolysaccharide (LPS) extracted from Porphyromonas gingivalis. hPDLFs and hPDLSCs were extracted from healthy young adults and were treated with EGCG and/or P. gingivalis LPS. After 1, 3, 5, and 7 days from treatment, cytotoxic and proliferative effects were evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and bromodeoxyuridine assay, respectively. And then, the gene expressions of hPDLFs and hPDLSCs were observed for interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL), and RANKL/OPG using real-time polymerase chain reaction (PCR) at 0, 6, 24, and 48 hours after treatment. The experiments were performed with the following groups for hPDLFs and hPDLSCs; 1) No treat, 2) EGCG alone, 3) P. gingivalis LPS alone, 4) EGCG+P. gingivalis LPS. The 20 µM of EGCG and 20 µg/mL of P. gingivalis LPS had the lowest cytotoxic effects, so those concentrations were used for further experiments. The proliferations of hPDLFs and hPDLSCs increased in all groups, though the 'EGCG alone' showed less increase. In real-time PCR, the hPDLFs and hPDLSCs of 'EGCG alone' showed similar gene expressions to those cells of 'no treat'. The gene expressions of 'P. gingivalis LPS alone' in both hPDLFs and hPDLSCs were highly increased at 6 hours for IL-1β, IL-6, TNF-α, RANKL, and RANKL/OPG, except the RANKL/OPG in hPDLSCs. However, those increased gene expressions were down-regulated in 'EGCG+P. gingivalis LPS' by the additional treatment of EGCG. Our results demonstrate that EGCG could exert an anti-inflammatory effect in hPDLFs and hPDLSCs against a major pathogen of periodontitis, P. gingivalis LPS.
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影响因子：
3.3
作者：
Bae, Jung Yoon;Kanamune, Jun;Hyon, Suong-Hyu
通讯作者：
Hyon, Suong-Hyu
影响因子：
64.8
作者：
GOWEN, M;WOOD, DD;RUSSELL, RGG
通讯作者：
RUSSELL, RGG
影响因子：
6.2
作者：
PFEILSCHIFTER, J;CHENU, C;ROODMAN, GD
通讯作者：
ROODMAN, GD
DOI：
10.1124/jpet.103.059220
发表时间：
2004-02-01
影响因子：
3.5
作者：
Ahmed, S;Wang, NZ;Haqqi, TM
通讯作者：
Haqqi, TM
影响因子：
4.2
作者：
Crespy, V;Williamson, G
通讯作者：
Williamson, G