Variation across a wheat genetic diversity panel for saccharification of hydrothermally pretreated straw.
Variation across a wheat genetic diversity panel for saccharification of hydrothermally pretreated straw.
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DOI：
10.1186/s13068-017-0914-x
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发表时间：
2017
影响因子：
6.3
通讯作者：
Waldron KW
中科院分区：
文献类型：
--
作者：
Collins SRA;Wilson DR;Moates GK;Harper AL;Bancroft I;Waldron KW
小麦秸秆形成用于第二代乙醇生产的木质纤维素生物质的重要且可靠的来源。然而，目前的育种品种秸秆质量的变化有有限的了解，这种变化的研究一般采用次优预处理。与确定良好反刍动物消化率的表型特征相比，在生物精炼的适当预处理后，与优化纤维素酶糖化相关的表型特征也存在一定程度的混淆。本研究的目的是（a）评估和比较优化水热预处理后从89个冬小麦品种获得的秸秆中酶促释放的葡萄糖水平和（B）确定与提高葡萄糖产量相关的潜在表型特征，特别是参考组成组织类型的比例。优化的预处理包括在210 °C下水热提取10分钟。使用过量的纤维素酶，在24小时内实现定量糖化。葡萄糖释放量范围为192 - 275 mg/g。葡萄糖释放的程度与（a）节间组织的水平相关（R = 0.498; p = 6.84 × 10−7），（B）股骨柄高度（R = 0.491; p = 1.03 × 10−6），以及（c）茎组织特有的化学特性，包括较高水平的纤维素（R = 0.552; p = 2.06 × 10−8）和更高水平的木质素R = 0.494; p = 8.67 × 10−7。为了实现用于第二代乙醇生产的纤维素葡萄糖的最大产量，小麦产生富含纤维素的节间茎组织（特别是较长长度的节间茎组织）的倾向将是有益的。这与反刍动物营养的理想类型形成对比，其中增加叶组织的比例是优选的。本文的在线版本（doi：10.1186/s13068-017-0914-x）包含补充材料，可供授权用户使用。
Wheat straw forms an important, reliable source of lignocellulosic biomass for use in second-generation ethanol production. However, there is limited understanding of the variation in quality of straw from current breeding cultivars, and studies on such variation have generally employed suboptimal pretreatments. There is also a degree of confusion regarding phenotypic characteristics relevant to optimising the enzymatic saccharification of cellulose after suitable pretreatments for biorefining compared with those which determine good ruminant digestibility. The aim of this study has been to (a) evaluate and compare the levels of glucose enzymatically released from straw obtained from 89 cultivars of winter wheat after optimised hydrothermal pretreatments and (b) identify the underlying phenotypic characteristics relevant to enhanced glucose production with special reference to the ratios of constituent tissue types. Optimised pretreatment involved hydrothermal extraction at 210 °C for 10 min. Using excess cellulases, quantitative saccharification was achieved within 24 h. The amount of glucose released ranged from 192 to 275 mg/g. The extent of glucose release was correlated with (a) the level of internode tissue (R = 0.498; p = 6.84 × 10−7), (b) stem height (R = 0.491; p = 1.03 × 10−6), and (c) chemical characteristics particular to stem tissues including higher levels of cellulose (R = 0.552; p = 2.06 × 10−8) and higher levels of lignin R = 0.494; p = 8.67 × 10−7. In order to achieve maximum yields of cellulosic glucose for second-generation ethanol production, a predisposition for wheat to produce cellulose-enriched internode stem tissue, particularly of longer length, would be beneficial. This contrasts with the ideotype for ruminant nutrition, in which an increased proportion of leaf tissue is preferable. The online version of this article (doi:10.1186/s13068-017-0914-x) contains supplementary material, which is available to authorized users.
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影响因子：
6.3
作者：
Elliston A;Wood IP;Soucouri MJ;Tantale RJ;Dicks J;Roberts IN;Waldron KW
通讯作者：
Waldron KW
影响因子：
6
作者：
Horn, Svein J.;Nguyen, Quang D.;Eijsink, Vincent G. H.
通讯作者：
Eijsink, Vincent G. H.
影响因子：
11.4
作者：
Elliston A;Collins SR;Wilson DR;Roberts IN;Waldron KW
通讯作者：
Waldron KW
影响因子：
4.4
作者：
Miller CN;Harper AL;Trick M;Werner P;Waldron K;Bancroft I
通讯作者：
Bancroft I
影响因子：
6
作者：
Lindedam, J.;Andersen, S. B.;Wyman, C. E.
通讯作者：
Wyman, C. E.