Drosophila Lysophospholipase Gene swiss cheese Is Required for Survival and Reproduction.
Drosophila Lysophospholipase Gene swiss cheese Is Required for Survival and Reproduction.
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果蝇溶血磷脂酶基因瑞士奶酪是生存和繁殖所必需的。
DOI：
10.3390/insects13010014
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发表时间：
2021-12-22
期刊：
影响因子：
3
通讯作者：
Sarantseva SV
中科院分区：
文献类型：
--
作者：
Melentev PA;Sharapenkov EG;Surina NV;Ivanova EA;Ryabova EV;Sarantseva SV
生物进化意味着由于基因突变而具有固有适应性特征的新进化生物体的适应性。然而，大多数突变都是有害的，它们破坏了生物体的生命、生存和繁衍后代的能力。有些基因对生物体来说是极其重要的，因此，它们倾向于保存自己的结构，不会突变，也不会很平静地突变。这就是编码PNPLA6溶血磷脂酶结构域的基因在细菌中进化的情况，进化迫使它在高等动物中保存其功能。在哺乳动物中，这种基因的完全功能障碍是致命的，因为它在胎盘中对早期胚胎发育非常重要。为什么在其他没有胎盘的物种中，比如昆虫，它是保守的？本研究研究了pnpla6编码基因swiss cheese在果蝇适应性中的作用。我们发现它的功能障碍导致标本过早死亡和它们不能留下足够的后代。因此，我们为编码溶血磷脂酶的基因在昆虫适应性中的重要性提供了第一个证据。果蝇（Drosophila melanogaster）是生物学研究中最著名的昆虫之一。它被广泛用于分析不同基因的功能。磷脂酰胆碱溶血磷脂酶基因最初被证明在果蝇神经系统中很重要。然而，该基因在非神经细胞类型中的作用尚未阐明，其功能保存的进化解释仍然难以捉摸。在这项研究中，我们分析了瑞士奶酪基因在黑腹果蝇健康中的表达模式和某些方面的作用。我们描述了瑞士奶酪在果蝇发育过程中的时空表达，并分析了瑞士奶酪突变体的生存和生产力。我们发现瑞士奶酪在唾液腺、中肠、马氏管、脂肪细胞和男性生殖系统中表达。瑞士奶酪功能障碍导致严重的蛹和象死亡率和生育力下降，这在雄性中是令人印象深刻的。后者伴有男性运动活动和求偶行为异常，睾丸囊肿细胞脂滴积聚，精子运动能力下降。这些结果表明，正常的瑞士奶酪对黑腹果蝇的健康很重要，因为它对果蝇的生存和繁殖成功都是必要的。
Biological evolution implies fitness of newly evolved organisms that have inherent adaptive traits because of mutations in genes. However, most mutations are detrimental, and they spoil the organism’s life, its survival and its ability to leave progeny. Some genes are extremely vital for an organism, and therefore, they tend to save their structure and do not mutate or do it very composedly. That is the case of the gene encoding PNPLA6 lysophospholipase domain that evolved in bacteria, and evolution obliged it to save its function in higher animals. In mammals, complete dysfunction of such a gene is lethal because of its high importance in placenta for early embryo development. Why is it conserved in other species, for instance insects, that have no placenta? Here we studied the role of the PNPLA6-encoding gene named swiss cheese in Drosophila melanogaster fitness. We have found that its dysfunction results in premature death of specimens and their inability to leave enough progeny. Thus, we provide the first evidence for significance of the gene that encodes the lysophospholipase enzyme in fitness of insects. Drosophila melanogaster is one of the most famous insects in biological research. It is widely used to analyse functions of different genes. The phosphatidylcholine lysophospholipase gene swiss cheese was initially shown to be important in the fruit fly nervous system. However, the role of this gene in non-nervous cell types has not been elucidated yet, and the evolutional explanation for the conservation of its function remains elusive. In this study, we analyse expression pattern and some aspects of the role of the swiss cheese gene in the fitness of Drosophila melanogaster. We describe the spatiotemporal expression of swiss cheese throughout the fly development and analyse the survival and productivity of swiss cheese mutants. We found swiss cheese to be expressed in salivary glands, midgut, Malpighian tubes, adipocytes, and male reproductive system. Dysfunction of swiss cheese results in severe pupae and imago lethality and decline of fertility, which is impressive in males. The latter is accompanied with abnormalities of male locomotor activity and courtship behaviour, accumulation of lipid droplets in testis cyst cells and decrease in spermatozoa motility. These results suggest that normal swiss cheese is important for Drosophila melanogaster fitness due to its necessity for both specimen survival and their reproductive success.
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影响因子：
2.4
作者：
delBarco-Trillo J;Mateo R;Roldan ER
通讯作者：
Roldan ER
乌龟精子发生过程中支持细胞内脂肪吞噬的新细胞证据
DOI：
10.18632/aging.101070
发表时间：
2016-10-16
期刊：
Aging
影响因子：
--
作者：
Ahmed N;Liu Y;Chen H;Yang P;Waqas Y;Liu T;Gandahi JA;Huang Y;Wang L;Song X;Rajput IR;Wang T;Chen Q
通讯作者：
Chen Q
影响因子：
21.3
作者：
Demarco, Rafael Senos;Uyemura, Bradley S.;Jones, D. Leanne
通讯作者：
Jones, D. Leanne
影响因子：
6
作者：
Deik A;Johannes B;Rucker JC;Sánchez E;Brodie SE;Deegan E;Landy K;Kajiwara Y;Scelsa S;Saunders-Pullman R;Paisán-Ruiz C
通讯作者：
Paisán-Ruiz C
影响因子：
64.5
作者：
Bailey AP;Koster G;Guillermier C;Hirst EM;MacRae JI;Lechene CP;Postle AD;Gould AP
通讯作者：
Gould AP