A new pulsed electric field therapy for melanoma disrupts the tumor's blood supply and causes complete remission without recurrence.
A new pulsed electric field therapy for melanoma disrupts the tumor's blood supply and causes complete remission without recurrence.
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一种针对黑色素瘤的新型脉冲电场疗法可破坏肿瘤的血液供应并导致完全缓解且不会复发
DOI：
10.1002/ijc.24345
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发表时间：
2009-07-15
影响因子：
6.4
通讯作者：
Schoenbach KH
中科院分区：
文献类型：
--
作者：
Nuccitelli R;Chen X;Pakhomov AG;Baldwin WH;Sheikh S;Pomicter JL;Ren W;Osgood C;Swanson RJ;Kolb JF;Beebe SJ;Schoenbach KH
我们发现了一种新的、超快速的治疗皮肤癌的方法，总电场暴露时间仅为180 μsec，效果非常好。”在体内对小鼠黑色素瘤施加300个高压（40 kV/cm）、超短（300 nsec）电脉冲，可触发坏死和凋亡，在17只接受治疗的动物中，平均47天内肿瘤完全缓解。这些黑色素瘤在最初的黑色素瘤消失后的4个月内没有复发。这些脉冲在暴露的细胞的质膜上产生小的、持久的、整流的纳米孔，导致膜对小分子和离子的渗透性增加，以及细胞内Ca2+的增加，DNA断裂，肿瘤血液供应的中断和细胞凋亡的开始。Bad标记增加3倍，Bcl-2标记减少72%表明细胞凋亡。此外，治疗后肿瘤内微血管密度下降93%。这种利用纳秒脉冲电场的新疗法具有高度局部靶向肿瘤细胞和总暴露时间仅为180 μsec的优点。这些脉冲穿透到每个肿瘤细胞的内部，引发DNA断裂和细胞凋亡，同时减少流向肿瘤的血流量。这种新的物理肿瘤治疗无需药物，高度局部化，能耗低，没有明显的副作用，而且疤痕很少。
We have discovered a new, ultrafast therapy for treating skin cancer that is extremely effective with a total electric field exposure time of only 180 μsec. The application of 300 high-voltage (40 kV/cm), ultrashort (300 nsec) electrical pulses to murine melanomas in vivo triggers both necrosis and apoptosis, resulting in complete tumor remission within an average of 47 days in the 17 animals treated. None of these melanomas recurred during a 4-month period after the initial melanoma had disappeared. These pulses generate small, long-lasting, rectifying nanopores in the plasma membrane of exposed cells, resulting in increased membrane permeability to small molecules and ions, as well as an increase in intracellular Ca2+, DNA fragmentation, disruption of the tumor’s blood supply and the initiation of apoptosis. Apoptosis was indicated by a 3-fold increase in Bad labeling and a 72% decrease in Bcl-2 labeling. In addition, microvessel density within the treated tumors fell by 93%. This new therapy utilizing nanosecond pulsed electric fields has the advantages of highly localized targeting of tumor cells and a total exposure time of only 180 μsec. These pulses penetrate into the interior of every tumor cell and initiate DNA fragmentation and apoptosis while at the same time reducing blood flow to the tumor. This new physical tumor therapy is drug free, highly localized, uses low energy, has no significant side effects and results in very little scarring.
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影响因子：
3.7
作者：
Al-Sakere B;André F;Bernat C;Connault E;Opolon P;Davalos RV;Rubinsky B;Mir LM
通讯作者：
Mir LM
影响因子：
5
作者：
Vernier, P. Thomas;Sun, Yinghua;Craviso, Gale L.
通讯作者：
Craviso, Gale L.
影响因子：
1.9
作者：
Pakhomov, Andrei G.;Kolb, Juergen F.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
3.9
作者：
Pakhomov, Andrei G.;Shevin, Rachael;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
2.8
作者：
Rubinsky, Boris
通讯作者：
Rubinsky, Boris