Tumor ablation with irreversible electroporation.
Tumor ablation with irreversible electroporation.
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DOI：
10.1371/journal.pone.0001135
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发表时间：
2007-11-07
期刊：
影响因子：
3.7
通讯作者：
Mir LM
中科院分区：
文献类型：
--
作者：
Al-Sakere B;André F;Bernat C;Connault E;Opolon P;Davalos RV;Rubinsky B;Mir LM
我们报告了第一次成功地使用不可逆电穿孔微创治疗侵袭性皮肤肿瘤植入小鼠。不可逆电穿孔是一种新开发的非热组织消融技术，其中使用某些短持续时间的电场来永久透化细胞膜，推测是通过在细胞膜中形成纳米级缺陷。在施加脉冲期间形成的电场和热场的数学模型用于设计具有最小组织加热的有效治疗方案。组织学研究证实了肿瘤消退，组织学研究还表明，肿瘤消退是不可逆细胞膜透化的直接结果。参数研究表明，手术的成功结果与所施加的电场强度、总脉冲持续时间以及脉冲输送的时间模式有关。我们的最佳结果是使用平板电极以0.3 Hz的频率在2500 V/cm的电场强度下通过肿瘤传递80个100 µs的脉冲。在不存在组织加热的情况下，这些条件在13个治疗的肿瘤中的12个（92%）中诱导完全消退。因此，不可逆电穿孔是一种新的有效的非热肿瘤消融模式。
We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 µs at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.
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影响因子：
8.4
作者：
MIR, LM;ORLOWSKI, S;PAOLETTI, C
通讯作者：
PAOLETTI, C
影响因子：
5.1
作者：
André, F;Mir, LM
通讯作者：
Mir, LM
影响因子：
3.4
作者：
Miklavcic, D;Beravs, K;Sersa, G
通讯作者：
Sersa, G
影响因子：
2.9
作者：
de Baere, Thierry;Abdel Rehim, Mohamed;Elias, Dominique
通讯作者：
Elias, Dominique
影响因子：
8.8
作者：
Mir, L M;Glass, L F;Sersa, G;Teissie, J;Domenge, C;Miklavcic, D;Jaroszeski, M J;Orlowski, S;Reintgen, D S;Rudolf, Z;Belehradek, M;Gilbert, R;Rols, M P;Belehradek, J Jr;Bachaud, J M;DeConti, R;Stabuc, B;Cemazar, M;Coninx, P;Heller, R
通讯作者：
Heller, R