Cellular apoptosis is associated with increased caveolin-1 expression in macrophages Published, JLR Papers in Press, June 1, 2003. DOI 10.1194/jlr.M300140-JLR200
Cellular apoptosis is associated with increased caveolin-1 expression in macrophages Published, JLR Papers in Press, June 1, 2003. DOI 10.1194/jlr.M300140-JLR200
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细胞凋亡与巨噬细胞中的 Caveolin-1 表达增加相关已发表，JLR Papers in Press，2003 年 6 月 1 日。DOI 10.1194/jlr.M300140-JLR200
作者：
Peter S. Gargalovic;L. Dory
巨噬细胞的凋亡是决定免疫反应效率、动脉粥样硬化病变稳定性和吞噬老化细胞清除能力的重要因素。小窝蛋白-1参与了成纤维细胞和上皮细胞的凋亡调控。在这里，我们表明，用各种无关的凋亡剂，包括辛伐他汀、喜树碱或葡萄糖剥夺来治疗硫代乙酸酯诱导的小鼠腹腔巨噬细胞，与小窝蛋白-1表达的特异性和大幅增加有关。相比之下，小窝蛋白-2的水平仍未受到影响。通过细胞形态的改变、膜联蛋白V标记和DNA片段化来检测细胞凋亡的诱导。我们证明，巨噬细胞中的小窝蛋白-1存在于脂筏中，并与磷脂酰丝氨酸(PS)共存于凋亡巨噬细胞的细胞表面。我们的数据表明，小窝蛋白-1的增加是一个早期事件，与PS外化密切相关，与caspase激活和核DNA片段化无关。小窝蛋白-1水平的增加不需要新的蛋白质合成，因为放线菌亚胺不能阻止细胞凋亡介导的小窝蛋白-1水平的增加。我们认为，小窝蛋白-1水平的增加是巨噬细胞凋亡表型的特征。Caveolin-1可能参与了PS在凋亡细胞表面的有效外化。
Macrophage apoptosis is an important factor in determining the efficiency of the immune response, atherosclerotic lesion stability, and clearance of aged cells by phagocytosis. The involvement of caveolin-1 in the regulation of apoptosis has been previously suggested in fibroblasts and epithelial cells. Here we show that treatment of thioglycollate-elicited mouse peritoneal macrophages with various unrelated apoptotic agents, including simvastatin, camptothecin, or glucose deprivation, is associated with a specific and large increase in caveolin-1 expression. In contrast, caveolin-2 levels remain unaffected. Induction of apoptosis was measured by changes in cell morphology, annexin V-labeling, and DNA fragmentation. We demonstrate that caveolin-1 in macrophages is present in lipid rafts and colocalizes with phosphatidylserine (PS) at the cell surface of apoptotic macrophages. Our data suggest that caveolin-1 increase is an early event, closely accompanied by PS externalization and independent of caspase activation and nuclear DNA fragmentation. The increase in caveolin-1 levels does not require new protein synthesis, as cycloheximide does not prevent the apoptosis-mediated increase in caveolin-1 levels. We propose that increased levels of caveolin-1 characterize the apoptotic phenotype of macrophages. Caveolin-1 may be involved in the efficient externalization of PS at the surface of the apoptotic cells.
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影响因子：
2.9
作者：
Pike, LJ;Han, XL;Gross, RW
通讯作者：
Gross, RW
影响因子：
3.9
作者：
Razani, B;Schlegel, A;Lisanti, MP
通讯作者：
Lisanti, MP
DOI：
--
发表时间：
1995-08
期刊：
The American journal of pathology
影响因子：
--
作者：
Y. Geng;P. Libby
通讯作者：
Y. Geng;P. Libby
影响因子：
20.3
作者：
Zhou, QS;Zhao, J;Sims, PJ
通讯作者：
Sims, PJ
DOI：
10.1073/pnas.92.5.1381
发表时间：
1995-02-28
影响因子：
11.1
作者：
KOLESKE, AJ;BALTIMORE, D;LISANTI, MP
通讯作者：
LISANTI, MP