Role of Heterotypic Neutrophil-in-Tumor Structure in the Prognosis of Patients With Buccal Mucosa Squamous Cell Carcinoma.
Role of Heterotypic Neutrophil-in-Tumor Structure in the Prognosis of Patients With Buccal Mucosa Squamous Cell Carcinoma.
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DOI：
10.3389/fonc.2020.541878
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发表时间：
2020
影响因子：
4.7
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Fan J;Fang Q;Yang Y;Cui M;Zhao M;Qi J;Luo R;Du W;Liu S;Sun Q
目的分析颊粘膜鳞状细胞癌（BMSCC）中异型嗜酸性结构（FNiT）的出现频率对患者预后的影响。 在体外，我们将BMSCC细胞系H157与中性粒细胞共培养，形成异型嗜中性粒细胞在肿瘤中的结构，然后对其进行荧光染色。临床上，回顾性入组了145例患者。采用卡方检验分析FNiT与年龄、性别、吸烟史、饮酒史、嚼槟榔史、肿瘤分期、淋巴结分期、转移、疾病分期、淋巴管浸润、淋巴结浸润、神经周围浸润和肿瘤分级等临床病理变量之间的关系。主要研究终点为无复发生存期（RFS）和疾病特异性生存期（DSS），采用Kaplan-Meier方法和考克斯模型。典型异型嗜中性粒细胞肿瘤结构的荧光染色结果显示，高分化的H157细胞比低分化的H157细胞具有更强的内化更多中性粒细胞的能力，后者通常仅内化一个中性粒细胞或不内化。平均FNiT为4.2‰，范围为2.3‰至7.8‰。共有80名患者复发，84名患者死于该病。5年RFS和DSS率分别为42%和42%。与FNiT <4.2 ‰的患者相比，FNiT ≥ 4.2 ‰的患者局部复发和癌症导致的死亡风险显著更高（分别为p=0.001和p<0.001）。单独的FNiT在预测RFS较差方面具有独立显着性，并且FNiT沿着肿瘤分级是DSS的独立预测因素。FNiT作为一种新的预测因子与BMSCC患者的RFS和DSS呈显著负相关。
To analyze the role of frequency of heterotypic neutrophil-in-tumor structure (FNiT) in the prognosis of patients with buccal mucosa squamous cell carcinoma (BMSCC). In vitro, we cocultured BMSCC cell line-H157 with neutrophils to form heterotypic neutrophil-in-tumor structures, which were then subject to fluorescence staining. Clinically, 145 patients were retrospectively enrolled. Associations between FNiT and clinicopathological variables including age, sex, smoking history, drinking history, betel nut chewing, tumor stage, node stage, metastasis, disease stage, lymphovascular invasion, extranodal extension, perineural invasion, and tumor grade were analyzed by chi-square test, and the main endpoints of interest were recurrence-free survival (RFS) and disease-specific survival (DSS) which were analyzed by the Kaplan-Meier method and Cox model. Fluorescent staining results of typical heterotypic neutrophil-in-tumor structure showed that well-differentiated H157 cells had a stronger ability to internalize more neutrophils than poorly-differentiated H157 cells, with the latter often internalizing only one neutrophil or nothing. The mean FNiT was 4.2‰, with a range from 2.3‰ to 7.8‰. A total of 80 patients relapsed and 84 patients died of the disease. The 5-year RFS and DSS rate was 42% and 42%, respectively. Patients with an FNiT≥4.2‰ had a significantly higher risk for locoregional recurrence and cancer-caused death than those with an FNiT<4.2‰ (p=0.001 and p<0.001, respectively). The FNiT alone was independently significant in predicting poor RFS, and the FNiT along with tumor grade was an independent predictor for DSS. The FNiT as a novel predictor is significantly negatively associated with both the RFS and DSS of patients with BMSCC.
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