Autoinducer production and quorum-sensing dependent phenotypes of Pseudomonas aeruginosa vary according to isolation site during colonization of intubated patients.
Autoinducer production and quorum-sensing dependent phenotypes of Pseudomonas aeruginosa vary according to isolation site during colonization of intubated patients.
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DOI：
10.1186/1471-2180-7-33
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发表时间：
2007-04-18
期刊：
影响因子：
4.2
通讯作者：
van Delden C
中科院分区：
文献类型：
--
作者：
Favre-Bonté S;Chamot E;Köhler T;Romand JA;van Delden C
铜绿假单胞菌经常定植并负责插管患者的严重呼吸机相关性肺炎。群体感应（QS）电路依赖于两种QS信号分子（自动诱导剂，AIs） 3-oxo-C12-HSL和C4-HSL的产生，调节铜绿假单胞菌几种毒力因子的产生，并且是生物膜形成所必需的。因此，qs抑制被认为是预防和/或治疗策略的新靶点。然而，QS在插管患者的定植和后续感染中的确切作用尚不清楚。我们想知道QS在插管患者的定植过程中是否活跃，以及在覆盖插管装置的生物膜内生长的铜绿假单胞菌分离株和在定植患者肺部居住的假单胞菌是否在其QS依赖性表型上有所不同。我们收集了8例铜绿假单胞菌患者的插管装置。我们在8台设备上检测到3-oxo-C12-HSL，在6台设备上检测到C4-HSL。在其中3例患者中，我们还从拔管时的气管吸附物中分离到了铜绿假单胞菌（n = 18），以及从插管装置中分离到了铜绿假单胞菌（n = 25）。我们对这些分离物进行了基因分型，量化了它们的AIs产量，并确定了三种依赖于qs的表型（粘附能力、生物膜和弹性酶产量）。来自插管装置的分离株的3-oxo-C12-HSL的产量持续增加，而来自气管吸入器的分离株的C4-HSL的产量明显更高。与气管吸入器分离的菌株相比，气管吸入器分离的菌株产生了大量的弹性蛋白酶，但生物膜较少，并且粘附能力略有降低。气管插管分离株的3-氧- c12 - hsl水平和弹性蛋白酶产生具有统计学相关性，而插管装置分离株的3-氧- c12 - hsl产生和粘附能力以及生物膜产生水平相关性较弱。我们的研究结果表明，在铜绿假单胞菌插管患者的定植过程中产生了自身诱导剂。铜绿假单胞菌生长的微环境可能会选择具有不同能力的细菌产生自诱导剂和某些依赖qs的表型。因此，qs抑制可能对生长在覆盖插管装置的生物膜内的分离株和居住在肺部的分离株产生不同的影响。
Pseudomonas aeruginosa frequently colonizes and is responsible for severe ventilator-associated pneumonia in intubated patients. A quorum-sensing (QS) circuit, depending on the production of the two QS-signaling molecules (autoinducers, AIs) 3-oxo-C12-HSL and C4-HSL, regulates the production by P. aeruginosa of several virulence factors and is required for biofilm formation. Therefore QS-inhibition has been suggested as a new target for preventive and/or therapeutic strategies. However the precise role of QS during colonization and subsequent infections of intubated patients remains unclear. We wondered whether QS is active during colonization of intubated patients, and whether P. aeruginosa isolates growing inside the biofilm covering the intubation devices and those resident in the lungs of colonized patients differ in their QS-dependent phenotypes. We collected the intubation devices of eight patients colonized by P. aeruginosa. We detected 3-oxo-C12-HSL on eight, and C4-HSL on six of these devices. In three of these patients we also obtained P. aeruginosa isolates from tracheal aspirates at the time of extubation (n = 18), as well as isolates from the intubation devices (n = 25). We genotyped these isolates, quantified their AIs production, and determined three QS-dependent phenotypes (adherence capacity, biofilm and elastase production). The production of 3-oxo-C12-HSL was consistently increased for isolates from the intubation devices, whereas the production of C4-HSL was significantly higher for isolates from tracheal aspirates. Isolates from tracheal aspirates produced significantly higher amounts of elastase but less biofilm, and had a marginally reduced adhesion capacity than isolates from the intubation devices. Levels of 3-oxo-C12-HSL and elastase production correlated statistically for tracheal intubation isolates, whereas levels of 3-oxo-C12-HSL production and adhesion ability, as well as biofilm production, correlated weakly amongst intubation device isolates. Our findings demonstrate that autoinducers are produced during the colonization of intubated patients by P. aeruginosa. The microenvironment, in which P. aeruginosa grows, may select for bacteria with different capacities to produce autoinducers and certain QS-dependent phenotypes. QS-inhibition might therefore affect differently isolates growing inside the biofilm covering intubation devices and those resident in the lungs.
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影响因子：
5.2
作者：
Favre-Bonté, S;Köhler, T;Van Delden, C
通讯作者：
Van Delden, C
影响因子：
9.4
作者：
Dénervaud, V;TuQuoc, P;van Delden, C
通讯作者：
van Delden, C
影响因子：
3.2
作者：
Cabrol, SN;Olliver, A;Ruimy, R
通讯作者：
Ruimy, R
影响因子：
2.1
作者：
Geisenberger, O;Givskov, M;Eberl, L
通讯作者：
Eberl, L
影响因子：
56.9
作者：
PASSADOR, L;COOK, JM;IGLEWSKI, BH
通讯作者：
IGLEWSKI, BH