Extracellular compartments in tendon morphogenesis: collagen fibril, bundle, and macroaggregate formation.
Extracellular compartments in tendon morphogenesis: collagen fibril, bundle, and macroaggregate formation.
复制标题
DOI：
10.1083/jcb.103.1.231
复制
发表时间：
1986-07
期刊：
影响因子：
--
通讯作者：
Trelstad RL
中科院分区：
文献类型：
--
作者：
Birk DE;Trelstad RL
利用常规电子显微镜和高压电子显微镜研究了鸡胚肌腱成纤维细胞形成胶原原纤维、原纤维束以及组织特异性胶原大聚集体的过程。在鸡肌腱形态发生过程中，至少存在三个细胞外区域，它们负责基质组织的三个层面：胶原原纤维、原纤维束以及胶原大聚集体。我们的观察结果表明，胶原原纤维形成过程中的初始细胞外事件发生在狭窄的细胞质凹陷内，可能是在细胞的紧密调控下进行的。胶原原纤维在这些深而狭窄的凹陷内形成，这些凹陷与细胞外空间相连通。当这些狭窄的凹陷与细胞表面融合时，细胞表面会因褶皱和突起而高度卷曲，这些褶皱和突起包裹着正在形成的原纤维束。原纤维束横向联合并融合，在第三个由细胞界定的细胞外区域内形成聚集体。我们的解释是，当细胞突起回缩且细胞质从原纤维束之间撤回时，形成了第三个区域。这些研究确定了肌腱内的一种层级结构，从原纤维组装延伸到肌束形成。细胞外不同层面的区域化与组织结构的相关性，为了解胶原原纤维及更高层次组装所涉及的细胞调控提供了见解，并且能更好地理解胶原原纤维是如何被聚集为结构群、定位以及编织成具有功能的组织特异性胶原大聚集体的。
The formation of collagen fibrils, fibril bundles, and tissue-specific collagen macroaggregates by chick embryo tendon fibroblasts was studied using conventional and high voltage electron microscopy. During chick tendon morphogenesis, there are at least three extracellular compartments responsible for three levels of matrix organization: collagen fibrils, bundles, and collagen macroaggregates. Our observations indicate that the initial extracellular events in collagen fibrillogenesis occur within narrow cytoplasmic recesses, presumably under close cellular regulation. Collagen fibrils are formed within these deep, narrow recesses, which are continuous with the extracellular space. Where these narrow recesses fuse with the cell surface, it becomes highly convoluted with folds and processes that envelope forming fibril bundles. The bundles laterally associate and coalesce, forming aggregates within a third cell-defined extracellular compartment. Our interpretation is that this third compartment forms as cell processes retract and cytoplasm is withdrawn between bundles. These studies define a hierarchical organization within the tendon, extending from fibril assembly to fascicle formation. Correlation of different levels of extracellular compartmentalization with tissue architecture provides insight into the cellular controls involved in collagen fibril and higher order assembly and a better understanding of how collagen fibrils are collected into structural groups, positioned, and woven into functional tissue-specific collagen macroaggregates.
登录
查看更多内容
DOI：
10.1016/0165-1838(83)90065-6
发表时间：
1983-01-01
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
CREUTZ, CE;POLLARD, HB
通讯作者：
POLLARD, HB
影响因子：
2.9
作者：
HONG, BS;DAVISON, PF;CANNON, DJ
通讯作者：
CANNON, DJ
影响因子：
2.9
作者：
KASTELIC, J;GALESKI, A;BAER, E
通讯作者：
BAER, E
DOI：
10.1098/rspb.1956.0011
发表时间：
1956-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JACKSON, SF
通讯作者：
JACKSON, SF
DOI：
10.1016/s0022-5320(67)80124-2
发表时间：
1967-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
GREENLEE, TK;ROSS, R
通讯作者：
ROSS, R