The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region.
The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region.
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Scarna2由独立的转录单元产生，其处理由编码区域指导。
DOI：
10.1093/nar/gkp988
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Carbon P
中科院分区：
文献类型：
--
作者：
Gérard MA;Myslinski E;Chylak N;Baudrey S;Krol A;Carbon P
预测C/D盒scaRNA 2指导U2 snRNA的特异性2′-O-甲基化。与其他SCARNA基因相反，SCARNA 2似乎是独立转录的。通过瞬时表达SCARNA 2-报告基因构建体，我们已经证明，该基因是由RNA聚合酶II转录的，并且负责其转录的启动子元件包含在转录起始位点上游的161 bp区域内。在哺乳动物中，我们已经确定了四个跨物种的保守启动子元件，TATA基序，hStaf/ZNF 143结合位点和两个新的元件，所需的完整的启动子活性。人hStaf/ZNF 143转录因子与其靶序列的结合是启动子活性所必需的，这表明hStaf/ZNF 143是SCARNA 2基因的基本调节因子。我们还发现，RNA聚合酶II继续转录超过3′-末端的成熟RNA，不管的Pol II启动子的身份。3′端的加工和积累由scaRNA 2编码区中包含的唯一信息控制，成熟通过与mRNA或snRNA产生不相容的特定途径发生。
The C/D box scaRNA2 is predicted to guide specific 2′-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter elements responsible for its transcription are contained within a 161 bp region upstream of the transcription start site. In mammals, we have identified four cross species conserved promoter elements, a TATA motif, an hStaf/ZNF143 binding site and two novel elements that are required for full promoter activity. Binding of the human hStaf/ZNF143 transcription factor to its target sequence is required for promoter activity, suggesting that hStaf/ZNF143 is a fundamental regulator of the SCARNA2 gene. We also showed that RNA polymerase II continues transcription past the 3′-end of the mature RNA, irrespective of the identity of the Pol II promoter. The 3′-end processing and accumulation are governed by the sole information contained in the scaRNA2 encoding region, the maturation occurring via a particular pathway incompatible with that of mRNA or snRNA production.
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影响因子：
3.5
作者：
Gerard, Marie-Aline;Krol, Alain;Carbon, Philippe
通讯作者：
Carbon, Philippe
影响因子：
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Medlin, JE;Uguen, P;Murphy, S
通讯作者：
Murphy, S
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ACH, RA;WEINER, AM
通讯作者：
WEINER, AM
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作者：
Myslinski E;Gérard MA;Krol A;Carbon P
通讯作者：
Carbon P
影响因子：
14.9
作者：
Myslinski, E;Amé, JC;Carbon, P
通讯作者：
Carbon, P