An unconventional myosin heavy chain gene from Drosophila melanogaster.
An unconventional myosin heavy chain gene from Drosophila melanogaster.
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来自果蝇的非常规的肌球蛋白重链基因。
DOI：
10.1083/jcb.119.4.823
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发表时间：
1992-11
影响因子：
7.8
通讯作者：
MILLER, KG
中科院分区：
文献类型：
--
作者：
KELLERMAN, KA;MILLER, KG
作为参与果蝇胚胎发育的细胞骨架蛋白研究的一部分，我们进行了140kD的ATP敏感肌动蛋白结合蛋白的分子分析(Miller，K.G.，C.M.field和B.M.Alberts)。1989年。J.细胞生物学。109：2963-2975)。对编码该蛋白的cDNA克隆的分析表明，该蛋白代表了一类新的非常规肌球蛋白重链。该蛋白的氨基末端三分之二包含一个与其他肌球蛋白头部29%-33%相同(60%-65%相似)的头部结构域，并含有ATP结合、肌动蛋白结合和钙调蛋白/肌球蛋白轻链结合基序。羧基末端的尾巴与其他已知的肌球蛋白尾巴没有明显的相似之处，但确实包含一个大约100个氨基酸的区域，据预测形成一个α-螺旋卷曲。由于编码该蛋白的唯一基因定位于多线图谱位置95F，我们将新基因命名为果蝇95F肌球蛋白重链(95F MHC)。95F MHC基因的表达谱复杂。对多个cDNA的检测表明，转录本是交替剪接的，至少编码三种蛋白质异构体；此外，在Western blotts上检测到第四种异构体。发育的Northern和Western杂交表明，转录本和蛋白质在整个生命周期中都存在，在胚胎发育中期和成年期出现表达高峰。在早期胚胎中的免疫定位表明，该蛋白主要以点状分布在周围细胞质中。大多数细胞在整个胚胎发育过程中保持低水平的蛋白质表达，但特定的组织似乎含有更多的蛋白质。我们推测，95F MHC蛋白亚型参与了果蝇发育的多个动态过程。
As part of a study of cytoskeletal proteins involved in Drosophila embryonic development, we have undertaken the molecular analysis of a 140-kD ATP-sensitive actin-binding protein (Miller, K. G., C. M. Field, and B. M. Alberts. 1989. J. Cell Biol. 109:2963-2975). Analysis of cDNA clones encoding this protein revealed that it represents a new class of unconventional myosin heavy chains. The amino-terminal two thirds of the protein comprises a head domain that is 29-33% identical (60-65% similar) to other myosin heads, and contains ATP-binding, actin-binding and calmodulin/myosin light chain-binding motifs. The carboxy-terminal tail has no significant similarity to other known myosin tails, but does contain a approximately 100-amino acid region that is predicted to form an alpha-helical coiled-coil. Since the unique gene that encodes this protein maps to the polytene map position 95F, we have named the new gene Drosophila 95F myosin heavy chain (95F MHC). The expression profile of the 95F MHC gene is complex. Examination of multiple cDNAs reveals that transcripts are alternatively spliced and encode at least three protein isoforms; in addition, a fourth isoform is detected on Western blots. Developmental Northern and Western blots show that transcripts and protein are present throughout the life cycle, with peak expression occurring during mid-embryogenesis and adulthood. Immunolocalization in early embryos demonstrates that the protein is primarily located in a punctate pattern throughout the peripheral cytoplasm. Most cells maintain a low level of protein expression throughout embryogenesis, but specific tissues appear to contain more protein. We speculate that the 95F MHC protein isoforms are involved in multiple dynamic processes during Drosophila development.
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DOI：
10.1083/jcb.109.6.2895
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Garcia A;Coudrier E;Carboni J;Anderson J;Vandekerkhove J;Mooseker M;Louvard D;Arpin M
通讯作者：
Arpin M
DOI：
10.1083/jcb.102.4.1494
发表时间：
1986-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Karr TL;Alberts BM
通讯作者：
Alberts BM
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
5.3
作者：
BERNSTEIN, SI;HANSEN, CJ;EMERSON, CP
通讯作者：
EMERSON, CP
影响因子：
7.5
作者：
CHENEY R E;MOOSEKER M S
通讯作者：
MOOSEKER M S