Organization of the cytoskeleton in early Drosophila embryos.
Organization of the cytoskeleton in early Drosophila embryos.
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DOI：
10.1083/jcb.102.4.1494
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发表时间：
1986-04
期刊：
影响因子：
--
通讯作者：
Alberts BM
中科院分区：
文献类型：
--
作者：
Karr TL;Alberts BM
早期，非细胞化的果蝇胚胎的细胞骨架已被检查的间接免疫荧光技术，使用整个坐骑可视化皮质细胞质和部分可视化内部。在核周期10完成向外的核迁移之前，肌动蛋白丝和微管都集中在几微米深的均匀表面层中，而微管网络包围着胚胎内部的每个核。早期胚胎中的这两个富含蛋白质的区域对应于细胞质的特殊区域，这些区域在组织切片的光学显微照片中倾向于排除细胞质颗粒。在内部的细胞核迁移到细胞表面并形成合胞胚盘后，可以看到每个细胞核被其自身的富含细丝的细胞质区域所包围，原始表面层的细胞骨架蛋白可能已被掺入其中。在间期，微管似乎是从每个核正上方的中心体组织起来的，延伸到整个细胞质皮质区（周质）至少40微米的深度。在细胞周期的这个阶段，每个细胞核上方的质膜下面也有一个肌动蛋白“帽”。当每个细胞核进入有丝分裂时，中心体分裂，微管重新排列形成有丝分裂纺锤体。位于质膜下的肌动蛋白展开，并且紧密间隔的相邻纺锤体被与连续的肌动蛋白亲和性丰富层相关的瞬时膜沟分开。因此，在合胞胚盘中的每个细胞核都被它自己的细胞质区域所包围，尽管它与数千个其他细胞核共享一个细胞质区室。
The cytoskeleton of early, non-cellularized Drosophila embryos has been examined by indirect immunofluorescence techniques, using whole mounts to visualize the cortical cytoplasm and sections to visualize the interior. Before the completion of outward nuclear migration at nuclear cycle 10, both actin filaments and microtubules are concentrated in a uniform surface layer a few micrometers deep, while a network of microtubules surrounds each of the nuclei in the embryo interior. These two filament-rich regions in the early embryo correspond to special regions of cytoplasm that tend to exclude cytoplasmic particles in light micrographs of histological sections. After the nuclei in the interior migrate to the cell surface and form the syncytial blastoderm, each nucleus is seen to be surrounded by its own domain of filament- rich cytoplasm, into which the cytoskeletal proteins of the original surface layer have presumably been incorporated. At interphase, the microtubules seem to be organized from the centrosome directly above each nucleus, extending to a depth of at least 40 microns throughout the cortical region of cytoplasm (the periplasm). During this stage of the cell cycle, there is also an actin "cap" underlying the plasma membrane immediately above each nucleus. As each nucleus enters mitosis, the centrosome splits and the microtubules are rearranged to form a mitotic spindle. The actin underlying the plasma membrane spreads out, and closely spaced adjacent spindles become separated by transient membrane furrows that are associated with a continuous actin filament-rich layer. Thus, each nucleus in the syncytial blastoderm is surrounded by its own individualized region of the cytoplasm, despite the fact that it shares a single cytoplasmic compartment with thousands of other nuclei.
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DOI：
10.1083/jcb.94.3.688
发表时间：
1982-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Manfredi JJ;Parness J;Horwitz SB
通讯作者：
Horwitz SB
DOI：
10.1083/jcb.100.5.1623
发表时间：
1985-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Foe VE;Alberts BM
通讯作者：
Alberts BM
影响因子：
2.7
作者：
FULLILOVE, SL;JACOBSON, AG
通讯作者：
JACOBSON, AG
DOI：
10.1007/bf01380620
发表时间：
1972-01-01
影响因子：
--
作者：
ILLMENSEE, K
通讯作者：
ILLMENSEE, K
影响因子：
1.5
作者：
Rabinowitz, M
通讯作者：
Rabinowitz, M