Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women.
Dysregulated microRNAs and their predicted targets associated with endometrioid endometrial adenocarcinoma in Hong Kong women.
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DOI：
10.1002/ijc.24071
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发表时间：
2009-03-15
影响因子：
6.4
通讯作者：
Wong YF
中科院分区：
文献类型：
--
作者：
Chung TK;Cheung TH;Huen NY;Wong KW;Lo KW;Yim SF;Siu NS;Wong YM;Tsang PT;Pang MW;Yu MY;To KF;Mok SC;Wang VW;Li C;Cheung AY;Doran G;Birrer MJ;Smith DI;Wong YF
本研究的目的是鉴定与子宫内膜样腺癌（EEC）相关的失调microRNAs（miRNAs），检测其与临床病理特征的相关性，并鉴定失调miRNAs的预测靶基因。使用实时定量逆转录-聚合酶链反应（qRT-PCR），在30个EEC和22个正常对应物中进行miRNA表达谱分析，其中全基因组基因表达先前已被分析和报告。聚类分析确定了30个在EEC中显著失调的miRNAs。一组miRNAs的表达与临床病理特征（包括分期、肌层浸润、复发和淋巴结转移）显著相关。通过搜索与先前鉴定的失调基因相关的预测的miRNA靶标，预测68个基因为这30个失调的miRNA的候选靶标。与正常对照相比，miR-205在EC中显著过表达。转染miR-205抑制剂后，miR-205在子宫内膜癌细胞系RL 95 -2中的表达降低，而其预测的靶基因JPH 4的蛋白表达增加。JPH 4是miR-205在体内外的一个真实的靶基因，也是EEC的一个候选抑癌基因。基于EEC中的这项研究，已经鉴定了预测参与肿瘤发生和肿瘤进展的miRNA，并将其置于EEC的转录组中。这项工作提供了一个框架，进一步研究新的诊断和治疗的EEC可以集中。
The objective of this study, a parallel study to global gene expression profiling, was to identify dysregulated microRNAs (miRNAs) associated with endometrioid endometrial adenocarcinoma (EEC), examine their correlation with clinico-pathological characteristics and identify predicted target genes of the dysregulated miRNAs. Using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), profiling of miRNA expression was performed in 30 EECs and 22 normal counterparts in which genome-wide gene expression had been previously profiled and reported. Clustering analysis identified 30 miRNAs which were significantly dysregulated in EEC. The expression of a sub-group of miRNAs was significantly correlated with clinico-pathological characteristics including stage, myometrial invasion, recurrence and lymph node involvement. By searching for predicted miRNA targets that were linked to the dysregulated genes previously identified, 68 genes were predicted as candidate targets of these 30 dysregulated miRNAs. miR-205 was significantly overexpressed in EECs compared with normal controls. After transfection of a miR-205 inhibitor, the expression of miR-205 in endometrial cancer cell line RL95-2 cells decreased whereas its predicted target gene, JPH4, showed increased protein expression. JPH4 seems to be a real miR-205 target in vitro and in vivo, and a candidate tumor suppressor gene in EEC. Based on this study in EEC, miRNAs predicted to be involved in tumorigenesis and tumor progression have been identified and placed in the context of the transcriptome of EEC. This work provides a framework on which further research into novel diagnosis and treatment of EEC can be focused.
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影响因子：
4.7
作者：
Boren, Todd;Xiong, Yin;Lancaster, Johnathan M.
通讯作者：
Lancaster, Johnathan M.
影响因子：
6.4
作者：
Lee, Eun Jon;Gusev, Yuriy;Schmittgen, Thomas D.
通讯作者：
Schmittgen, Thomas D.
DOI：
10.1073/pnas.0703942104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Gironella, Meritxell;Seux, Mylene;Dusetti, Nelson J.
通讯作者：
Dusetti, Nelson J.
影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM
影响因子：
11.4
作者：
Debernardi, S.;Skoulakis, S.;Young, B. D.
通讯作者：
Young, B. D.