Proteoglycan and collagen expression during human air conducting system development.
Proteoglycan and collagen expression during human air conducting system development.
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DOI：
10.4081/ejh.2012.e29
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发表时间：
2012-06-29
期刊：
影响因子：
--
通讯作者：
Pereda J
中科院分区：
文献类型：
--
作者：
Godoy-Guzmán C;San Martin S;Pereda J
肺是由芽形成的，芽以两种方式生长和分裂。芽是一种新的生长中心，由上皮-间质相互作用决定，细胞外基质（ECM）蛋白可能参与其中。为了了解这种蛋白质在人体肺部发育过程中的作用，我们检查了在安装空气传导系统期间与不同类型胶原蛋白相关的蛋白聚糖的表达和分布。利用光镜和免疫组化技术，我们评估了受精后8 ~ 10周和胎龄11 ~ 14周的人胚胎和胎儿肺中胶原（I、III和VI）和蛋白聚糖（decorin、biglycan和lumican）的表达。我们发现decorin， lumican和所有类型的胶原蛋白都在上皮-间质界面表达，在细支气管导管周围形成一个套筒。此外，biglycan在血管内皮细胞和平滑肌中均有表达。因此，胶原蛋白和蛋白多糖在人肺早期发育阶段的相似分布模式可能与支气管树的二分分化过程密切相关。本研究提供了关于在空气传导系统安装在人胎儿肺期间胶原和蛋白多糖参与上皮-间质界面的新见解。
The lung is formed from a bud that grows and divides in a dichotomous way. A bud is a new growth center which is determined by epithelial-mesenchymal interactions where proteins of the extracellular matrix (ECM) might be involved. To understand this protein participation during human lung development, we examined the expression and distribution of proteoglycans in relation to the different types of collagens during the period in which the air conducting system is installed. Using light microscopy and immunohistochemistry we evaluate the expression of collagens (I, III and VI) and proteoglycans (decorin, biglycan and lumican) between 8 to 10 weeks post fertilization and 11 to 14 weeks of gestational age of human embryo and fetus lungs. We show that decorin, lumican and all the collagen types investigated were expressed at the epithelium-mesenchymal interface, forming a sleeve around the bronchiolar ducts. In addition, biglycan was expressed in both the endothelial cells and the smooth muscle of the blood vessels. Thus, the similar distribution pattern of collagen and proteoglycans in the early developmental stages of the human lung may be closely related to the process of dichotomous division of the bronchial tree. This study provides a new insight concerning the participation of collagens and proteoglycans in the epithelial-mesenchymal interface during the period in which the air conducting system is installed in the human fetal lung.
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影响因子：
4.3
作者：
CHENG, F;HEINEGARD, D;FRANSSON, LA
通讯作者：
FRANSSON, LA
影响因子：
2.4
作者：
Giachini, F. R. C.;Carriel, V.;Martin, S. San
通讯作者：
Martin, S. San
DOI：
10.1164/ajrccm/144.2.452
发表时间：
1991-08-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
LWEBUGAMUKASA, JS
通讯作者：
LWEBUGAMUKASA, JS
影响因子：
3
作者：
McEwan, Paul A.;Scott, Paul G.;Bella, Jordi
通讯作者：
Bella, Jordi
影响因子：
7.3
作者：
Kaarteenaho-Wiik, R;Pääkkö, P;Soini, Y
通讯作者：
Soini, Y