Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene.
Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene.
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DOI：
10.1093/nar/gkj501
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发表时间：
2006
影响因子：
14.9
通讯作者：
Waters R
中科院分区：
文献类型：
--
作者：
Ferreiro JA;Powell NG;Karabetsou N;Mellor J;Waters R
研究了野生型、gcn 5 Δ和ada 2 Δ酿酒酵母细胞在阻遏和去阻遏条件下的染色质结构、MET 16基因环丁烷嘧啶二聚体的转录和修复。佐贺/ADA染色质重塑复合物的这两种组分对于MET 16的基础转录是消耗性的，但对于其完全转录诱导是必需的。尽管它们对转录的影响，但两种蛋白质都不会引起MET 16染色质结构的重大变化，但会发生一些轻微的变化。在所有菌株和任一生长条件下，转录链编码区的修复比非转录区的修复更快。此外，转录的MET 16越多，修复越快。数据显示，通过改变转录程度，每条DNA链的修复速率以不同的方式改变，证实该位点的修复受到其染色质结构和转录水平的强烈调节。GCN 5或ADA 2的缺失减少了MET 16时的修复。结果进行了讨论，根据目前的理解Gcn 5 p和Ada 2 p的功能，他们是第一个报告的作用Ada 2 p在核苷酸切除修复的调控和转录区的基因。
Chromatin structure, transcription and repair of cyclobutane pyrimidine dimers at the MET16 gene of wild type, gcn5Δ and ada2Δ Saccharomyces cerevisiae cells were studied under repressing or derepressing conditions. These two components of the SAGA/ADA chromatin remodelling complexes are expendable for the basal transcription of MET16 but are mandatory for its full transcription induction. Despite their influence on transcription neither protein induces major changes in MET16 chromatin structure, but some minor ones occur. Repair at the coding region of the transcribed strand is faster than repair at non-transcribed regions in all strains and either growth condition. Moreover, the more MET16 is transcribed the faster the repair. The data show that by changing the transcription extent the rate of repair at each DNA strand is altered in a different way, confirming that repair at this locus is strongly modulated by its chromatin structure and transcription level. Deletion of GCN5 or ADA2 reduces repair at MET16. The results are discussed in light of the current understanding of Gcn5p and Ada2p functions, and they are the first to report a role for Ada2p in the nucleotide excision repair of the regulatory and transcribed regions of a gene.
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影响因子：
3.8
作者：
Powell, NG;Ferreiro, J;Waters, R
通讯作者：
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