Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction.
Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction.
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DOI：
10.1172/jci.insight.170518
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发表时间：
2023-12-08
期刊：
影响因子：
8
通讯作者：
Fry, Jean L.
中科院分区：
文献类型：
--
作者：
Wen, Yuan;Latham, Christine M.;Moore, Angelique N.;Thomas, Nicholas T.;Lancaster, Brooke D.;Reeves, Kelsey A.;Keeble, Alexander R.;Fry, Christopher S.;Johnson, Darren L.;Thompson, Katherine L.;Noehren, Brian;Fry, Jean L.
尽管已知25-羟基维生素D [25（OH）D]浓度为30 ng/mL或更高可降低损伤风险并提高强度，但对前交叉韧带重建（ACLR）结果的影响尚未得到研究。本研究旨在确定维生素D信号对ACLR的反应，评估维生素D状态与肌纤维横截面积（CSA）和骨密度结果之间的关系，并发现ACLR后维生素D受体（VDR）的靶点。入组了21例近期ACL撕裂的年轻、健康、体力活动活跃的受试者（17.8 ± 3.2岁，BMI 26.0 ± 3.5 kg/m2）。在基线、ACLR后1周、4个月和6个月，通过血液样本、股外侧肌活检、双能X线骨密度测量和等速测力计测量收集数据。活检有助于CSA、蛋白质印迹、RNA-seq和VDR ChIP-seq分析。ACLR手术导致ACLR后1周循环生物活性维生素D减少，VDR和骨骼肌活化酶表达增加。25（OH）D水平低于30 ng/mL的受试者（n = 13）在ACLR后1周和4个月表现出比30 ng/mL或更高水平的受试者更显著的股四头肌纤维CSA损失（n = 8;事后比较P < 0.01;时间×维生素D状态相互作用P = 0.041）。RNA-seq和ChIP-seq数据整合揭示了与能量代谢和骨骼肌恢复相关的基因，可能介导维生素D状态对ACLR恢复的影响。未观察到组间骨密度损失的差异。在ACLR之前纠正维生素D状态可能有助于在恢复期间保护骨骼肌。NIH赠款R 01 AR 072061、R 01 AR 071398 - 04 S1和K99 AR 081367。
Although 25-hydroxyvitamin D [25(OH)D] concentrations of 30 ng/mL or higher are known to reduce injury risk and boost strength, the influence on anterior cruciate ligament reconstruction (ACLR) outcomes remains unexamined. This study aimed to define the vitamin D signaling response to ACLR, assess the relationship between vitamin D status and muscle fiber cross-sectional area (CSA) and bone density outcomes, and discover vitamin D receptor (VDR) targets after ACLR. Twenty-one young, healthy, physically active participants with recent ACL tears were enrolled (17.8 ± 3.2 years, BMI 26.0 ± 3.5 kg/m2). Data were collected through blood samples, vastus lateralis biopsies, dual energy x-ray bone density measurements, and isokinetic dynamometer measures at baseline, 1 week, 4 months, and 6 months after ACLR. The biopsies facilitated CSA, Western blotting, RNA-seq, and VDR ChIP-seq analyses. ACLR surgery led to decreased circulating bioactive vitamin D and increased VDR and activating enzyme expression in skeletal muscle 1 week after ACLR. Participants with less than 30 ng/mL 25(OH)D levels (n = 13) displayed more significant quadriceps fiber CSA loss 1 week and 4 months after ACLR than those with 30 ng/mL or higher (n = 8; P < 0.01 for post hoc comparisons; P = 0.041 for time × vitamin D status interaction). RNA-seq and ChIP-seq data integration revealed genes associated with energy metabolism and skeletal muscle recovery, potentially mediating the impact of vitamin D status on ACLR recovery. No difference in bone mineral density losses between groups was observed. Correcting vitamin D status prior to ACLR may aid in preserving skeletal muscle during recovery. NIH grants R01AR072061, R01AR071398-04S1, and K99AR081367.
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