Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells.
Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells.
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DOI：
10.4049/jimmunol.0801973
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发表时间：
2009-02-15
期刊：
影响因子：
--
通讯作者：
Estes DM
中科院分区：
文献类型：
--
作者：
Endsley MA;Njongmeta LM;Shell E;Ryan MW;Indrikovs AJ;Ulualp S;Goldblum RM;Mwangi W;Estes DM
在过去的几年里，关于树突状细胞(DC)在B细胞激活和调节中的作用的研究已经取得了很大的进展。树突状细胞分泌的趋化因子可诱导B细胞向淋巴结转移。DC产生的生存因子，如BAFF和APRIL，已被证明是B细胞成熟所必需的，但也与类别转换重组和B细胞淋巴瘤的生存有关。最近添加到这个影响B细胞的DC衍生因子名单中的是IgA诱导蛋白(IGIP)。在此，我们鉴定了人DC产生IGIP的特性，并检测了其在体外诱导幼稚B细胞IgA类转换和分化的能力。体外培养单核细胞来源的DC，加入TLR激动剂(3，4，5，9)和其他因子，包括CD40L，GM-CSF，IL-4和神经肽血管活性肠肽(VIP)。在VIP和CD40L的体外刺激下，IGIP mRNA的表达在12-48小时内可上调35倍。与外源重组人免疫球蛋白一起培养的幼稚B细胞比未刺激的对照组产生更多的IgA。最后，我们证明了免疫球蛋白刺激驱动免疫球蛋白M+/免疫球蛋白+幼稚的人B细胞产生μ-α开关环，表明其作为免疫球蛋白开关因子的作用。
Over the last several years there has been a great deal of progress in characterizing the role of dendritic cells (DCs) in the activation and modulation of B cells. DC-secreted chemokines can induce B cell trafficking to the lymph nodes. DC-produced survival factors such as BAFF and APRIL have been shown to be essential for B cell maturation, but have also been implicated in class-switch recombination and B cell lymphoma survival. Recently added to this list of DC-derived factors effecting B cells is IgA-inducing protein (IGIP). Here we characterize production of IGIP by human DCs, and examine its capacity to induce IgA class switching and differentiation of naïve B cells in vitro. Monocyte derived DCS were cultured in vitro with TLR agonists (3,4,5, and 9) and other factors, including CD40L, GM-CSF, and IL-4 as well as the neuropeptide vasoactive intestinal peptide (VIP). Under in vitro stimulation with VIP and CD40L, IGIP mRNA expression could be up-regulated as much as thirty five-fold above non-stimulated samples within 12–48 hours. Naïve B cells cultured with exogenous rhIGIP produced IgA in greater quantities than non-stimulated controls. Finally, we demonstrate that IGIP stimulation drives the production of μ-α switch circles from IgM+/IgD+ naïve human B cells, indicating its role as an IgA switch factor.
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影响因子：
5.5
作者：
Delgado, M;Reduta, A;Ganea, D
通讯作者：
Ganea, D
影响因子：
7.4
作者：
Moon, Eun-Yi;Lee, Jun-Hee;Yoon, Won-Kee
通讯作者：
Yoon, Won-Kee
影响因子：
56.9
作者：
ARPIN, C;DECHANET, J;LIU, YJ
通讯作者：
LIU, YJ
DOI：
10.1111/j.1749-6632.1995.tb44446.x
发表时间：
1995-01-01
期刊：
COMBINED VACCINES AND SIMULTANEOUS ADMINISTRATION
影响因子：
--
作者：
EHRHARDT, RO;STROBER, W
通讯作者：
STROBER, W
影响因子：
20.3
作者：
KIMATA, H;FUJIMOTO, M
通讯作者：
FUJIMOTO, M