Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.
Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.
复制标题
小鼠体内脑代谢的无创光声计算机断层扫描。
DOI：
10.1016/j.neuroimage.2012.08.054
复制
发表时间：
2013-01-01
期刊：
影响因子：
5.7
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Yao, Junjie;Xia, Jun;Maslov, Konstantin I.;Nasiriavanaki, Mohammadreza;Tsytsarev, Vassiliy;Demchenko, Alexei V.;Wang, Lihong V.
关键词：
我们已经证明了使用光声计算机断层扫描（PACT），一种快速，非侵入性和功能性的成像方式与光学对比度和声学分辨率成像小鼠脑代谢的可行性。对前爪刺激的脑反应经皮和经颅成像。2-NBDG在血脑屏障中扩散良好，为葡萄糖反应的光声成像提供了外源性对比。同时，血红蛋白为血流动力学反应的光声成像提供内源性对比。葡萄糖和血流动力学反应定量解耦使用双波长测量。我们发现，葡萄糖摄取和血液灌注周围的躯体感觉区域的对侧半球都增加了刺激，表明神经元活动的升高。虽然葡萄糖反应区更均匀，并局限于躯体感觉区，血液动力学反应区有一个明确的血管模式，比躯体感觉区更广泛。我们的研究结果表明，2-NBDG增强的PACT是一个很有前途的工具，脑代谢的非侵入性研究。
We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood-brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively decoupled by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area had a clear vascular pattern and spread wider than the somatosensory region. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.
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影响因子：
3.1
作者：
O'Neil, RG;Wu, L;Mullani, N
通讯作者：
Mullani, N
影响因子：
20.6
作者：
Li, Meng-Lin;Oh, Jung-Taek;Wang, Lihong V.
通讯作者：
Wang, Lihong V.
DOI：
10.1523/jneurosci.0026-12.2012
发表时间：
2012-06-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hall CN;Klein-Flügge MC;Howarth C;Attwell D
通讯作者：
Attwell D
影响因子：
8.6
作者：
Fang, Hui;Maslov, Konstantin;Wang, Lihong V.
通讯作者：
Wang, Lihong V.
影响因子：
4.8
作者：
Mehagnoul-Schipper, DJ;van der Kallen, BFW;Jansen, RWMM
通讯作者：
Jansen, RWMM