A fragile metabolic network adapted for cooperation in the symbiotic bacterium Buchnera aphidicola.
A fragile metabolic network adapted for cooperation in the symbiotic bacterium Buchnera aphidicola.
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DOI：
10.1186/1752-0509-3-24
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发表时间：
2009-02-21
影响因子：
--
通讯作者：
Douglas AE
中科院分区：
文献类型：
--
作者：
Thomas GH;Zucker J;Macdonald SJ;Sorokin A;Goryanin I;Douglas AE
在计算机方面，分析提供了对专性细胞内病原体和具有小基因组的共生体的生物学的有价值的见解。现在有一个特别的机会，可以应用为模式细菌大肠杆菌开发的系统级工具来研究共生菌的进化和功能，这些共生菌在代谢上专门为宿主过度生产特定的营养物质，并且值得注意的是，它们具有作为大肠杆菌基因组子集的基因互补。我们已经重建和分析了γ蛋白细菌的代谢网络(豌豆蚜虫的共生体)，作为使用系统水平的方法来发现具有小基因组的共生体的关键特征的模型。新陈代谢网络极其脆弱，有90%的反应对硅胶的生存至关重要；它的结构使得细菌在不产生必要的氨基酸组氨酸的情况下无法生长，组氨酸被释放给昆虫宿主。此外，细菌在硅胶中产生的必需氨基酸的数量可以通过宿主提供的碳和氮底物来控制。这一系统水平的分析预测，细菌代谢网络的脆弱性使共生细菌不能耐受剧烈的环境波动，而组氨酸生产与生长的耦合阻止细菌在不交互的情况下利用宿主营养。这些代谢特性支持了蜂蜜杆菌对寄主的持续营养贡献，再加上寄主来源的底物对细菌释放的营养物质的影响，表明寄主在控制共生中发挥着主导作用。
In silico analyses provide valuable insight into the biology of obligately intracellular pathogens and symbionts with small genomes. There is a particular opportunity to apply systems-level tools developed for the model bacterium Escherichia coli to study the evolution and function of symbiotic bacteria which are metabolically specialised to overproduce specific nutrients for their host and, remarkably, have a gene complement that is a subset of the E. coli genome. We have reconstructed and analysed the metabolic network of the γ-proteobacterium Buchnera aphidicola (symbiont of the pea aphid) as a model for using systems-level approaches to discover key traits of symbionts with small genomes. The metabolic network is extremely fragile with > 90% of the reactions essential for viability in silico; and it is structured so that the bacterium cannot grow without producing the essential amino acid, histidine, which is released to the insect host. Further, the amount of essential amino acid produced by the bacterium in silico can be controlled by host supply of carbon and nitrogen substrates. This systems-level analysis predicts that the fragility of the bacterial metabolic network renders the symbiotic bacterium intolerant of drastic environmental fluctuations, whilst the coupling of histidine production to growth prevents the bacterium from exploiting host nutrients without reciprocating. These metabolic traits underpin the sustained nutritional contribution of B. aphidicola to the host and, together with the impact of host-derived substrates on the profile of nutrients released from the bacteria, point to a dominant role of the host in controlling the symbiosis.
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