Dynamic RNA-Seq Study Reveals the Potential Regulators of Seed Germination in Paris polyphylla var. yunnanensis.
Dynamic RNA-Seq Study Reveals the Potential Regulators of Seed Germination in Paris polyphylla var. yunnanensis.
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动态 RNA 测序研究揭示了重楼种子萌发的潜在调节因子。
DOI：
10.3390/plants11182400
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发表时间：
2022-09-15
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影响因子：
--
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中科院分区：
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多叶巴黎变种云南红豆杉是一种重要的中药材，但种子发芽率低限制了其大规模人工栽培。因此，了解重楼种子萌发的调控因子，对提高巴黎人工栽培技术水平具有重要意义。在本研究中，使用处于三个萌发阶段的种子进行RNA测序，包括未萌发的种子（阶段1）、胚根长度为0.5 cm的萌发种子（阶段2）和温暖层积（20 °C）90天后胚根长度为2.0 cm的萌发种子（阶段3）。在种子萌发过程中获得了大约2.2亿个干净的读数和447，314个注释的unigenes，其中在阶段1至阶段2、阶段1至阶段3和阶段2至阶段3分别鉴定了总共4454、5150和1770个差异表达基因（DEG）。基因和基因组百科全书（KEGG）分析表明，DEG显着丰富的碳水化合物代谢，脂质代谢，信号转导和翻译。其中，几个基因编码的谷氨酸脱羧酶，谷氨酰胺合成酶，α-半乳糖苷酶，生长素反应蛋白IAA 30，脱落酸反应元件结合因子，促分裂原活化蛋白激酶激酶9/18，和小和大亚基核糖体蛋白被确定为潜在参与种子萌发。这些基因为研究巴黎种子萌发的分子基础提供了宝贵的资源。云南种。
Paris polyphylla var. yunnanensis is an important traditional Chinese medicine, but poor seed germination limits its large-scale artificial cultivation. Thus, it is crucial to understand the regulators of seed germination to obtain clues about how to improve the artificial cultivation of Paris polyphylla. In this study, the seeds at three germination stages, including ungerminated seeds (stage 1), germinated seeds with a 0.5 cm radicel length (stage 2), and germinated seeds with a 2.0 cm radicel length (stage 3) after warm stratification (20 °C) for 90 days were used for RNA sequencing. Approximately 220 million clean reads and 447,314 annotated unigenes were obtained during seed germination, of which a total of 4454, 5150, and 1770 differentially expressed genes (DEGs) were identified at stage 1 to stage 2, stage 1 to stage 3, and stage 2 to stage 3, respectively. Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEGs were significantly enriched in carbohydrate metabolism, lipid metabolism, signal transduction, and translation. Of them, several genes encoding the glutamate decarboxylase, glutamine synthetase, alpha-galactosidase, auxin-responsive protein IAA30, abscisic-acid-responsive element binding factor, mitogen-activated protein kinase kinase 9/18, and small and large subunit ribosomal proteins were identified as potentially involved in seed germination. The identified genes provide a valuable resource to study the molecular basis of seed germination in Paris polyphylla var. yunnanensis.
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