A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity.
A Geographically Diverse Collection of Schizosaccharomyces pombe Isolates Shows Limited Phenotypic Variation but Extensive Karyotypic Diversity.
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DOI：
10.1534/g3.111.001123
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Warringer J
中科院分区：
文献类型：
--
作者：
Brown WR;Liti G;Rosa C;James S;Roberts I;Robert V;Jolly N;Tang W;Baumann P;Green C;Schlegel K;Young J;Hirchaud F;Leek S;Thomas G;Blomberg A;Warringer J
裂殖酵母已被广泛用于研究真核细胞生物学，但几乎所有的工作都使用了单一菌株的衍生物。我们研究了81个粟酒裂殖酵母的独立自然菌株和3个指定的实验室菌株。粟酒裂殖酵母在大小上变化很大，但与酿酒酵母相比，在不同环境中的增殖变化有限。核苷酸多样性，π，在近中性位点，染色体II的着丝粒的中央核心约为0.7%。通过脉冲场凝胶电泳和滤膜杂交分析，约20%的分离株表现出核型重排。一个易位，发现在6个不同的分离株，包括模式菌株，具有地理上广泛的分布和独特的单倍型，并可能是一个早期的物种形成事件的标记。所有其他易位都是独特的。这种核型多样性的开发利用可能会对端粒和着丝粒的生物学以及单细胞真核生物的分离机制产生新的影响。
The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, π, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.
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影响因子：
64.8
作者：
Schacherer J;Shapiro JA;Ruderfer DM;Kruglyak L
通讯作者：
Kruglyak L
影响因子：
4.5
作者：
Nogami S;Ohya Y;Yvert G
通讯作者：
Yvert G
影响因子：
64.8
作者：
Nordborg, Magnus;Weigel, Detlef
通讯作者：
Weigel, Detlef
DOI：
10.1073/pnas.0803466105
发表时间：
2008-07-08
影响因子：
11.1
作者：
Lynch, Michael;Sung, Way;Thomas, W. Kelley
通讯作者：
Thomas, W. Kelley
影响因子：
56.9
作者：
Fournier-Level, A.;Korte, A.;Wilczek, A. M.
通讯作者：
Wilczek, A. M.