Micro-algae come of age as a platform for recombinant protein production.
Micro-algae come of age as a platform for recombinant protein production.
复制标题
DOI：
10.1007/s10529-010-0326-5
复制
发表时间：
2010-10
影响因子：
2.7
通讯作者：
Mayfield, Stephen
中科院分区：
文献类型：
--
作者：
Specht, Elizabeth;Miyake-Stoner, Shigeki;Mayfield, Stephen
一套完整的遗传工具仍在开发中的微型衣原体reinhardtii。然而，即使有这个不完整的集合，这种光合作用的单细胞植物已经证明了作为重组蛋白表达平台的重要前景。近年来，已经开发了允许来自核基因组和质体基因组的基因的稳健表达的技术。随着这些进展，许多研究小组已经研究了这种和其他微藻作为能够生产重组肽和蛋白质的生物机器的柔韧性。这篇综述介绍了最近成功的重组蛋白生产衣原体，包括生产复杂的哺乳动物治疗蛋白和单克隆抗体的水平足以生产在经济平价与现有的生产平台。这些进展还揭示了分子水平上藻类蛋白质生产的细节，并为优化微藻作为生产治疗和工业相关重组蛋白的有用平台的下一步提供了见解。
A complete set of genetic tools is still being developed for the micro-alga Chlamydomonas reinhardtii. Yet even with this incomplete set, this photosynthetic single-celled plant has demonstrated significant promise as a platform for recombinant protein expression. In recent years, techniques have been developed that allow for robust expression of genes from both the nuclear and plastid genome. With these advances, many research groups have examined the pliability of this and other micro-algae as biological machines capable of producing recombinant peptides and proteins. This review describes recent successes in recombinant protein production in Chlamydomonas, including production of complex mammalian therapeutic proteins and monoclonal antibodies at levels sufficient for production at economic parity with existing production platforms. These advances have also shed light on the details of algal protein production at the molecular level, and provide insight into the next steps for optimizing micro-algae as a useful platform for the production of therapeutic and industrially relevant recombinant proteins.
登录
查看更多内容
影响因子：
--
作者：
Chebolu, S.;Daniell, H.
通讯作者：
Daniell, H.
影响因子：
2.6
作者：
Griesbeck, Christoph;Kobl, Iris;Heitzer, Markus
通讯作者：
Heitzer, Markus
影响因子：
4.9
作者：
Abe, Jun;Hiwatashi, Yuji;Sekimoto, Hiroyuki
通讯作者：
Sekimoto, Hiroyuki
影响因子：
4.4
作者：
Chen, F;Johns, MR
通讯作者：
Johns, MR
DOI：
10.1073/pnas.0711310105
发表时间：
2008-04-29
影响因子：
11.1
作者：
Casas-Mollano, J. Armando;Jeong, Byeong-Ryool;Cerutti, Heriberto
通讯作者：
Cerutti, Heriberto