Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda.
Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda.
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DOI：
10.1371/journal.pone.0063413
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Joloba ML
中科院分区：
文献类型：
--
作者：
Kateete DP;Kabugo U;Baluku H;Nyakarahuka L;Kyobe S;Okee M;Najjuka CF;Joloba ML
Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic infections, isolates from milkmen are also described. Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew Staphylococcus aureus. Furthermore, 24 (25%) coliforms namely Escherichia coli (12), Klebsiella oxytoca (5), Proteus vulgaris (2), Serratia (2), Citrobacter (1), Cedecea (1) and Leclercia (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four Staphylococcus aureus. Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant Staphylococcus aureus was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows (Staphylococcus hominis, Staphylococcus lugdunensis) and humans (Staphylococcus scuiri). Also, vancomycin and daptomycin resistant Enterococci (Enterococcus faecalis and Enterococcus faecium, respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant Klebsiella, Proteus, Serratia, Cedecea, and Citrobacter were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine Staphylococcus aureus were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns. CoNS, Enterococci, Streptococci, and Escherichia coli are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected.
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从不同欧洲国家中分离出的细菌病原体中抗菌素耐药性的患病率：2002- 2004年。
影响因子：
2.1
作者：
Hendriksen, Rene S.;Mevius, Dik J.;Schroeter, Andreas;Teale, Christopher;Meunier, Daniele;Butaye, Patrick;Franco, Alessia;Utinane, Andra;Amado, Alice;Moreno, Miguel;Greko, Christina;Staerk, Katharina;Berghold, Christian;Myllyniemi, Anna-Liisa;Wasyl, Dariusz;Sunde, Marianne;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.
影响因子：
1.8
作者：
Kateete DP;Namazzi S;Okee M;Okeng A;Baluku H;Musisi NL;Katabazi FA;Joloba ML;Ssentongo R;Najjuka FC
通讯作者：
Najjuka FC
影响因子：
3.6
作者：
Hagnestam-Nielsen, C.;Ostergaard, S.
通讯作者：
Ostergaard, S.
DOI：
10.1099/00207713-37-3-257
发表时间：
1987-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
DEVRIESE, LA;VANDEKERCKHOVE, A;SCHLEIFER, KH
通讯作者：
SCHLEIFER, KH
影响因子：
5.7
作者：
Huijsdens, Xander W;van Dijke, Beatrix J;de Neeling, Albert J
通讯作者：
de Neeling, Albert J